METABOLISM STUDIES OF E COLI

大肠杆菌的代谢研究

• 批准号: 7960022
• 负责人: JOEL T SMITH
• 金额: $ 7.62万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960022
• 关键词: Amino Acids; Biological Assay; Biological Models; Biomedical Research; Capillary Electrophoresis; Carbohydrates; Chemicals; Collaborations; Complex Mixtures; Computer Retrieval of Information on Scientific Projects Database; Disease; Ecosystem; Enterobacteriaceae; Escherichia coli; Funding; Goals; Grant; Habitats; In Vitro; Individual; Institution; Intestines; Knowledge; Metabolism; Methodology; Methods; Monitor; Mucous body substance; Mus; Nutrient; Oklahoma; Process; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Universities; base; gastrointestinal; in vivo; in vivo Model; pathogen; preference; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this bioanalytical project is to: 1) develop capillary electrophoresis-based assays capable of resolving complex mixtures of carbohydrates and 2) apply the methodologies to analyze nutrient availability in a gastrointestinal ecosystem of biomedical importance. More specifically, the in vivo nutrient content of mouse intestinal mucus, which is the habitat for infecting E. coli, will be examined. This project will be a collaborative effort between Dr. Tyrrell Conway of the University of Oklahoma and Dr. Tim Smith at Southeastern Oklahoma State University. Mutational analysis in mouse colonization studies revealed several sugars that appeared to be important for E. coli to colonize. An important extension of these findings is to use chemical methods to prove these sugars are available in mucus and to determine E. coli's in vivo order of preference for the individual mucus-derived sugars. In this study, capillary electrophoresis was utilized to monitor the carbohydrate and amino acid metabolism of various E. coli strains using both in vitro and in vivo models. The goal is to determine the actual nutrient availability to enteric bacteria in the intestine. This collaboration is providing a deeper understanding of how gastrointestinal pathogens acquire the nutrients necessary to infect their host and begin the disease process and should build on the knowledge of this model system.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该生物分析项目的具体目标是：1）开发能够解析复杂碳水化合物混合物的基于毛细管电泳的测定方法，2）应用该方法分析具有生物医学重要性的胃肠道生态系统中的营养物质可用性。 更具体地说，在体内的营养含量的小鼠肠粘液，这是栖息地感染E。大肠杆菌进行检测。 这个项目将是俄克拉荷马州大学的泰瑞尔·康威博士和东南俄克拉荷马州州立大学的蒂姆·史密斯博士之间的合作。 小鼠定殖研究中的突变分析揭示了几种糖对E.大肠杆菌定植。 这些发现的一个重要延伸是使用化学方法来证明这些糖在粘液中是可用的，并确定E。大肠杆菌对单个粘液衍生糖的体内偏好顺序。 本研究利用毛细管电泳技术对不同菌株的碳水化合物和氨基酸代谢进行了研究。大肠杆菌菌株使用体外和体内模型。 目的是确定肠道细菌的实际营养可用性。 这项合作提供了一个更深入的了解胃肠道病原体如何获得必要的营养物质感染他们的主机和开始疾病的过程，并应建立在这个模型系统的知识。