NEUROBIOLOGICAL/CARCINOGENETIC EFFECTS OF ELECTROPOLLUTION & HAZARDOUS CHEMICALS

电污染的神经生物学/致癌作用

• 批准号: 6239908
• 负责人: SISIR K DUTTA
• 金额: $ 4.83万
• 依托单位: HOWARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6239908
• 关键词: cell transformation; chemical carcinogen; chemical carcinogenesis; electromagnetic radiation; environmental contamination; human genetic material tag; human tissue; neurobiology; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; radiation dosage; radiation genetics; radiation hazard; radiation sensitivity; radiobiology; tissue /cell culture; western blottings

The primary objectives of this project will be provide motivation and training in human health effects of electropollution (electric and magnetic fields radiations) and carcinogenic substances using molecular genetics approaches to one undergraduate and two graduate students through different ongoing biomedical projects of Dr. S.K. Dutta. Graduate students, currently working in the MBRS project will continue their studies for 2 to 4 years. Undergraduate students will be expected to continue their studies for 15 to 24 months. Students will be trained in library studies, in collection and analysis of data for presentations of papers in meetings and for publications. The involvement of students in the three currently funded ongoing projects will be in the areas of: (i) Pathogenesis of neuron specific enolase (NSE), which is involved in the glycolytic pathway of neuron cells induced by electropollution. The possible alterations of expression of cloned NSE genes and of chick embryo retina cells by electropollution will also be studied. One graduate student will do thesis work in this project. (ii) The second area relates to construction of DNA probes, containing catabolic genes capable to biodegrade toxic organic chemicals in the environment, for rapid screening of vast numbers of known and unknown soil microorganisms as well as soil DNAs from contaminated sites. One undergraduate student will be involved in these screening studies using DNA probes followed by analytical studies for the expression of genes for biodegradation. (iii) Another funded project (United Nation's Developmental Fund) is in the area of developing DNA diagnostic tools as biomarkers for early detection of haemoglobin pathogenesis and cancer incidence. One graduate student will do thesis work in these studies of detecting B-thalassemic mutations in patients using nonradioactive DNA labeling and PCR (polymerase chain reaction) technology. Two MBRS graduate students are expected to write M.S. and/or Ph.D. theses on their ongoing research areas.

该项目的主要目标将是提供动力和 关于电污染对人体健康影响的培训(电和 磁场辐射)和使用分子的致癌物质 一名本科生和两名研究生的遗传学方法 通过S.K.杜塔博士正在进行的不同生物医学项目。 目前在MBRS项目工作的研究生们将继续 他们的学习时间为2至4年。预计将有本科生 继续他们的学习15到24个月。学生们将接受培训 在图书馆学中，为陈述而收集和分析数据 会议和出版物中的论文。 目前正在资助的三个项目的学生参与 项目将在以下领域开展：(I)神经元特异性致病机制 参与神经元糖酵解途径的烯醇化酶(NSE) 由电污染引起的细胞。可能发生的变化 克隆的NSE基因在鸡胚视网膜细胞中的表达 电子污染也将被研究。一个研究生就可以了 本项目中的论文工作。(Ii)第二个范畴与建造有关 含有能够生物降解有毒物质的分解代谢基因的DNA探针 环境中的有机化学品，用于快速筛选大量 已知和未知的土壤微生物以及来自 受污染的场地。一名本科生将参与这些活动 使用DNA探针筛选研究，然后进行分析研究 生物降解基因的表达。(Iii)另一项获资助的项目 (联合国发展基金)在开发DNA方面 作为早期检测血红蛋白的生物标志物的诊断工具 发病机制和癌症发病率。一名研究生将撰写论文 从事检测患者B-地中海贫血突变的研究 利用非放射性DNA标记和聚合酶链式反应 技术两名MBR研究生需要写出硕士学位和/或 关于他们正在进行的研究领域的博士论文。