ISOLATION AND LOCALIZATION OF KINESIN IN PARAMECIUM

分泌、溶酶体的形成和受体介导的内吞作用 都是通过囊泡包装和运输来调节的。细胞器内 沟通还涉及囊泡的运输。其中一些 转运过程利用核苷酸敏感的微管结合 为微管上的小泡提供能量的蛋白质。水泡和水泡 草履虫吞噬系统中的空泡已被证明是 沿着至少三个不同的明确定义的微管运输 系统，横跨口腔区域的口腔微管丝带和 细胞直肠，后纤维由许多束微管和 细胞直肠及邻近基底部的微管 身体。这些先前研究的合乎逻辑的延伸是表征 马达(S)负责这三个动作。因为我们已经净化了 一组马达，细胞质动力蛋白，我们建议研究 可能存在第二组马达--激动素 草履虫。我们将提纯激动素和针对单抗的 激动素，以确定该马达在细胞中的位置并进行探针 其在体外和体内的功能。关于正常细胞如何 指导和调节囊泡运输是这项提案的目标。

Secretion, the formation of lysosomes and receptor-mediated endocytosis are all mediated by vesicular packaging and transport. Intraorganelle communication also involves the transport of vesicles. Some of these transport processes utilize nucleotide-sensitive microtubule-binding proteins to power the vesicles along microtubules. The vesicle and vacuole in the phagosomal system in Paramecium have been shown to be transported along at least three different well defined microtubule systems, the oral microtubular ribbons that span the oral region and the cytoproct, the postoral fiber consisting many bundles of microtubules and the microtubules arising from the cytoproct and the adjacent basal bodies. A logical extension of these previous studies is to characterize the motor(s) responsible for the three movements. As we have purified one group of motors, the cytoplasmic dyneins, we proposed to investigate the possibility that kinesin, a second group of motors, may be present in Paramecium. We will purified kinesin and to monoclonal antibodies to kinesin to determine the location of this motor in the cell and to probe its in vitro and in vivo functions. New information on how normal cells direct and regulate vesicular transport is the goal of this proposal.