PREPARATION OF GONADAL CELLS AND CELL FRACTIONS

性腺细胞和细胞组分的制备

• 批准号: 2407562
• 负责人: NORMAN N BEAUDRY
• 金额: $ 24.5万
• 依托单位: COVANCE LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-12-11 至 1997-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2407562
• 关键词: animal tissue; cell components; gonads; hormone receptor; tissue /cell culture; tissue /cell preparation

The program on analysis of gonadotropin receptors and actions of gonadotropic hormones is an important component of the Endocrinology and Reproduction Research Branch scientific research objectives. This program is supported by a Contract that provides large amounts of well characterized preparations including purified gonadal cells, various short term and established cell cultures, gonadal tissues and cell fractions, and receptor preparations. Gonadal cells are prepared acutely from a large number of control animals and under multiple protocols. The contract supports work in reproductive biology allowing investigators to perform studies on the binding characteristics of gonadotropin and prolactin receptors of testicular, ovarian, Nb2 lymphoma and several transfected cells, and cell membranes; and of the biological characterization of LH and related hormones, and studies on the mechanisms of hormone-receptor interaction, signal transduction, interaction between signalling pathways, and modulation of key metabolic steps of the androgen biosynthetic pathways.

促性腺激素受体分析程序及其作用 促性腺激素是内分泌学的重要组成部分， 生殖研究分会的科研目标。本节目 得到了一份提供大量油井的合同的支持 特征制剂包括提纯的性腺细胞，各种短的 成熟和成熟的细胞培养，性腺组织和细胞组分， 和受体制剂。性腺细胞是从一种尖锐的 大量的控制动物，并在多种协议下。这个 合同支持生殖生物学方面的工作，允许研究人员 研究促性腺激素和促性腺激素的结合特性 睾丸、卵巢、NB2淋巴瘤和几种淋巴瘤的催乳素受体 转基因细胞和细胞膜；以及生物的 黄体生成素及其相关激素的特性及其作用机制的研究 激素-受体相互作用，信号转导，相互作用 信号通路和雄激素关键代谢步骤的调节 生物合成途径。