MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
批准号:
2672918
负责人:
H. G. Archie Bouwer
金额:
$16.97万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-07-01 至 2001-06-30
关键词:
Listeria MHC class I antigen antibacterial agents antigen presentation bacterial antigens bacterial proteins bactericidal immunity cell line cellular immunity cytotoxic T lymphocyte laboratory mouse mutant passive immunization peptide chemical synthesis protein sequence tissue /cell culture virulence
中文摘要
描述(改编自申请人摘要):保护性免疫
回应L。单核细胞增多症用于研究细胞介导的免疫
转化为细胞内细菌 一个必要的毒力决定因素是分泌
溶藻素O(LLO)。 李斯特菌特异性CD 8 + T细胞在
在体外,这些效应物裂解L.单核细胞增多症感染的目标。 在BALB/c
小鼠(H2 d),已经分离出Kd限制性的CD 8 + CTL,
对应于LLO的aa 91 -99的纳米聚体肽显示由
MHC Ia类分子。 还描述了LLO特异性CD 8 + T细胞
其裂解L.单核细胞增多症感染的MHC IB类限制性靶点
抗原呈递分子编码在免疫球蛋白的T区内。
较少多态性的MHC I类分子家族。 目标1中的研究将
确定LLO是否是MHC Ib类限制性抗原的免疫显性抗原
抗肿瘤CTL。 目标2中的研究将确定MHC Ib类是否
受限制的抗卵泡细胞在体内具有保护性。 研究目的3
将鉴定LLO衍生的MHC Ib类限制性CTL表位。 结果
从这些研究将提供一个理解的作用,MHC类Ib
限制性效应细胞在保护性免疫反应中的作用
胞内病原体
英文摘要
DESCRIPTION (Adapted from the applicant's abstract): The protective immune
response to L. monocytogenes is used to investigate cell-mediated immunity
to intracellular bacteria. A necessary virulence determinant is secretion
of listeriolysin O (LLO). Listeria-specific CD8+ T cells are cytotoxic in
vitro, these effectors lyse L. monocytogenes infected targets. In BALB/c
mice (H2d), CD8+ CTLs have been isolated which are Kd restricted, and a
nanomer peptide corresponding to aa91-99 of LLO shown to be presented by the
MHC Class Ia molecule. LLO-specific CD8+ T-cells have also been described
which lyse L. monocytogenes infected targets in an MHC Class IB restricted
manner; the antigen presenting molecule is encoded within the T-region of a
less polymorphic family of MHC Class I molecules. Studies in Aim 1 will
determine if LLO is an immuno-dominant antigen for MHC Class Ib restricted
antilisterial CTLs. Studies in Aim 2 will determine if MHC Class Ib
restricted anti-listerial cells are protective in vivo. Studies in Aim 3
will identify the LLO-derived MHC Class Ib-restricted CTL epitope. Results
from these studies will provide an understanding of the role of MHC Class Ib
restricted effector cell in the protective immune response to an
intracellular pathogen.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6374036
-
项目类别:
-
资助金额:$18.3万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6170947
-
项目类别:
-
资助金额:$17.77万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:2898534
-
项目类别:
-
资助金额:$18.39万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6532768
-
项目类别:
-
资助金额:$17.75万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6647219
-
项目类别:
-
资助金额:$19.4万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:2887360
-
项目类别:
-
资助金额:$17.48万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:2388624
-
项目类别:
-
资助金额:$16.48万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:6169777
-
项目类别:
-
资助金额:$18.01万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
ANIMAL FACILITY OF CHILES RESEARCH INSTITUTE
-
批准号:3059282
-
项目类别:
-
资助金额:$10.0万
-
财政年份:1992
-
负责人:H. G. Archie Bouwer
-
依托单位:
海外基金