MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
批准号:
2672918
负责人:
H. G. Archie Bouwer
金额:
$16.97万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-07-01 至 2001-06-30
关键词:
Listeria MHC class I antigen antibacterial agents antigen presentation bacterial antigens bacterial proteins bactericidal immunity cell line cellular immunity cytotoxic T lymphocyte laboratory mouse mutant passive immunization peptide chemical synthesis protein sequence tissue /cell culture virulence
中文摘要
描述(改编自申请人的摘要):保护性免疫
对单核细胞增多性李斯特菌的反应用于研究细胞免疫
细胞内的细菌。一个必要的毒力决定因素是分泌物。
李斯特菌溶血素O(LLO)。李斯特菌特异性CD8+T细胞具有细胞毒作用
在体外,这些效应物能溶解L.单核细胞增多症的感染靶点。在BALB/c中
已分离出KD限制性的小鼠(H2d)、CD8+CTL,并且a
与LLO的aa91-99相对应的纳米肽由
MHC Ia类分子。也描述了L10特异性的CD8+T细胞
单核细胞增多性乳杆菌在MHC类IB限制性感染靶点中的作用
抗原提呈分子被编码在T区内
多态较少的MHC I类分子家族。目标1中的研究将
确定LLO是否是MHC Class Ib限制性的免疫显性抗原
抗李斯特菌CTL。AIM 2的研究将确定MHC Ib类物质是否
限制性抗李氏杆菌细胞在体内具有保护性。AIM 3中的研究
将确定LLO衍生的MHC Ib类限制性CTL表位。结果
这些研究将提供对MHC Ib类的作用的理解
限制性效应细胞在保护性免疫反应中的作用
细胞内病原体。
英文摘要
DESCRIPTION (Adapted from the applicant's abstract): The protective immune
response to L. monocytogenes is used to investigate cell-mediated immunity
to intracellular bacteria. A necessary virulence determinant is secretion
of listeriolysin O (LLO). Listeria-specific CD8+ T cells are cytotoxic in
vitro, these effectors lyse L. monocytogenes infected targets. In BALB/c
mice (H2d), CD8+ CTLs have been isolated which are Kd restricted, and a
nanomer peptide corresponding to aa91-99 of LLO shown to be presented by the
MHC Class Ia molecule. LLO-specific CD8+ T-cells have also been described
which lyse L. monocytogenes infected targets in an MHC Class IB restricted
manner; the antigen presenting molecule is encoded within the T-region of a
less polymorphic family of MHC Class I molecules. Studies in Aim 1 will
determine if LLO is an immuno-dominant antigen for MHC Class Ib restricted
antilisterial CTLs. Studies in Aim 2 will determine if MHC Class Ib
restricted anti-listerial cells are protective in vivo. Studies in Aim 3
will identify the LLO-derived MHC Class Ib-restricted CTL epitope. Results
from these studies will provide an understanding of the role of MHC Class Ib
restricted effector cell in the protective immune response to an
intracellular pathogen.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6374036
-
项目类别:
-
资助金额:$18.3万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6170947
-
项目类别:
-
资助金额:$17.77万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:2898534
-
项目类别:
-
资助金额:$18.39万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6532768
-
项目类别:
-
资助金额:$17.75万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
EPITOPE DELIVERY, IMMUNODOMINANCE AND VACCINE EFFICACY
-
批准号:6647219
-
项目类别:
-
资助金额:$19.4万
-
财政年份:1999
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:2887360
-
项目类别:
-
资助金额:$17.48万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:2388624
-
项目类别:
-
资助金额:$16.48万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
MHC CLASS IB IMMUNITY TO AN INTRACELLULAR PATHOGEN
-
批准号:6169777
-
项目类别:
-
资助金额:$18.01万
-
财政年份:1997
-
负责人:H. G. Archie Bouwer
-
依托单位:
ANIMAL FACILITY OF CHILES RESEARCH INSTITUTE
-
批准号:3059282
-
项目类别:
-
资助金额:$10.0万
-
财政年份:1992
-
负责人:H. G. Archie Bouwer
-
依托单位:
海外基金