ESTROGENIC EFFECTS IN ANDROGEN SENSITIVE MOTONEURONS

描述：雌激素被认为是主要的代谢物 负责大脑性别二型性区域的分化。在……里面 相比之下，脊髓中性二形核的发育 一直被认为受到雄激素的严格控制。测试 这种明显的二分法在批判形式中的有效性和局限性 类固醇激素与神经系统的性分化 而他们行动地点的潜在差异是这一点的焦点 求婚。在大鼠中使用简单的激素敏感神经肌肉系统 脊髓，将检查该系统的三个不同特征： 运动神经元形态(组织化学)、临界神经元的分布 酶(免疫细胞化学)和神经肌肉功能 (电生理学)。雌激素和雄激素的协同作用， 雌激素可获得性的必要性、脊柱上和局部的作用 神经元间、雌激素敏感的传入及其对自然的影响 将评估形态发育过程中发生的雌激素水平。 因为芳香酶的瞬时表达已经在各种不同的 对于发育中的神经结构，出生后的脊髓将被检测 芳香酶的存在。雌激素在调节血管紧张素转换酶中的作用 在这些运动神经元中常见的缝隙连接的发展将 也要接受检查。运动神经元在发育过程中兴奋性的变化 可能是脊椎介导的反射的发生的原因，以及 雌激素和雄激素在几种功能特性发育中的作用 对这些激素敏感的运动神经元进行评估。因为最近 证据表明，雌激素实际上与脊髓有关。 男性化，这些研究可能有助于统一我们对 发育中的哺乳动物中枢的性别分化过程 神经系统。

DESCRIPTION: Estrogen is considered to be the primary metabolite responsible for the differentiation of sexually dimorphic brain regions. In contrast, the development of sexually dimorphic nuclei in the spinal cord has been considered to be regulated strictly by androgens. Testing the validity and limitations of this apparent dichotomy in the critical forms of steroid hormones involved in sexual differentiation of the nervous system and potential differences in their site of action is the focus of this proposal. Using a simple hormone-sensitive neuromuscular system in the rat spinal cord, three different features of this system will be examined: aspects of motoneuron morphology (histochemistry), distribution of critical enzymes (immunocytochemistry), and neuromuscular function (electrophysiology). Synergistic effects of estrogen and androgen, the necessity of estrogen availability, the role of supraspinal and local interneuronal, estrogen-sensitive afferents, and the effects of naturally occurring estrogen levels in morphological development will be assessed. Because the transient expression of aromatase has been reported in a variety of developing neural structures, postnatal spinal cords will be assayed for the presence of aromatase. The role of estrogen in regulating the development of gap junctions, which are common in these motoneurons, will also be examined. Changes in motoneuron excitability during development are likely responsible for the onset of spinally mediated reflexes, and the role of estrogen and androgen in the development of several functional properties of these hormone-sensitive motoneurons will be assessed. Because recent evidence suggests that estrogen is in fact involved in spinal cord masculinization, these studies may help in unifying our understanding of the process of sexual differentiation across the developing mammalian central nervous system.