LH/CG RECEPTOR REGULATION IN THE OVARY

促黄体生成素/绒毛膜促性腺激素受体(LHR)起着关键作用 在生殖生理学中的作用。在女性身上，它的表达在 卵巢对排卵和初始排卵都是必需的。 维持妊娠。在卵泡发育、排卵、 黄体生成和黄体溶解LHR水平差异很大，通常 在复杂的荷尔蒙控制下。LHR HAS基因的克隆 允许我们的实验室以及其他S检测LHR mRNA的水平 伴随着卵巢中LHR数量的已知变化。已经展示了T 事实上，受体数量和受体数量之间存在着非常密切的关联 MRNA水平，提示转录和/或后转录的调节。 与LHR基因相关的转录事件在 LHR受体数量的调节。最近，我们已经能够 促性腺激素(CAMP)介导的促性腺激素释放激素(LHR) 大鼠颗粒细胞分化至少部分是由一种 LHR基因转录增加。初步实验 还表明在5‘侧翼区域内的一个或多个区域 LHR基因介导了cAMP的这种刺激作用。这些实验 在此提出的是旨在进一步解决分子机制 大鼠卵巢促性腺激素释放激素的调控 系统。 具体来说，我们建议： 1.确定参与的顺式和反式作用因素 LHR基因在卵巢中的表达。 2.测定卵泡刺激素(CAMP)对颗粒细胞LHR基因表达的影响。 3.测定LHR基因转录和LHR mRNA的相对变化 都是已知发生的。

The luteinizing hormone/choriogonadotropin receptor (LHR) plays a pivotal role in reproductive physiology. In the female, its expression in the ovary is necessary both for ovulation as well as for the initial maintenance of pregnancy. During follicular development, ovulation, luteinization, and luteolysis the levels of LHR vary greatly and are often under complex hormonal control. The cloning of the cDNA for the LHR has allowed our lab as well as other s to examine the levels of LHR mRNA which accompany known changes in LHR numbers in the ovary. t has been shown that there is in fact a very tight correlation between receptor numbers and mRNA levels, suggesting that regulation of transcriptional and/or post- transcriptional events related to the LHR gene are critical in the modulation of LHR receptor numbers. More recently, we have been able to demonstrate that the FSH(cAMP)-mediated increase in LHR mRNA in differentiating rat granulosa cells is mediated at least in part by an increase in the transcription of the LHR gene. Preliminary experiments also indicate that one or more regions within the 5'flanking region of the LHR gene mediate this stimulatory effect of cAMP. The experiments proposed herein are designed to further address the molecular mechanisms underlying the regulation of the LHR in the ovary, using the rat as a model system. Specifically, we propose to: 1. Determine the cis- and trans-acting factors which are involved in the ovarian expression of the LHR gene. 2. Determine whether FSH(cAMP)) stabilizes LHR mRNA in granulosa cells. 3. Determine the relative changes in LHR gene transcription and LHR mRNA are known to occur.