LH/CG RECEPTOR REGULATION IN THE OVARY
LH/CG RECEPTOR REGULATION IN THE OVARY
批准号:
2668602
负责人:
DEBORAH SEGALOFF
金额:
$17.35万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-03-01 至 2000-02-29
关键词:
DNA footprinting chorionic gonadotropin female follicle stimulating hormone gel mobility shift assay genetic regulatory element granulosa cell hormone receptor hormone regulation /control mechanism laboratory rat luteinizing hormone nuclear runoff assay ovary receptor expression transcription factor
中文摘要
促黄体生成素/绒毛膜促性腺激素受体(LHR)起着关键作用
在生殖生理学中的作用。在女性身上,它的表达在
卵巢对排卵和初始排卵都是必需的。
维持妊娠。在卵泡发育、排卵、
黄体生成和黄体溶解LHR水平差异很大,通常
在复杂的荷尔蒙控制下。LHR HAS基因的克隆
允许我们的实验室以及其他S检测LHR mRNA的水平
伴随着卵巢中LHR数量的已知变化。已经展示了T
事实上,受体数量和受体数量之间存在着非常密切的关联
MRNA水平,提示转录和/或后转录的调节。
与LHR基因相关的转录事件在
LHR受体数量的调节。最近,我们已经能够
促性腺激素(CAMP)介导的促性腺激素释放激素(LHR)
大鼠颗粒细胞分化至少部分是由一种
LHR基因转录增加。初步实验
还表明在5‘侧翼区域内的一个或多个区域
LHR基因介导了cAMP的这种刺激作用。这些实验
在此提出的是旨在进一步解决分子机制
大鼠卵巢促性腺激素释放激素的调控
系统。
具体来说,我们建议:
1.确定参与的顺式和反式作用因素
LHR基因在卵巢中的表达。
2.测定卵泡刺激素(CAMP)对颗粒细胞LHR基因表达的影响。
3.测定LHR基因转录和LHR mRNA的相对变化
都是已知发生的。
英文摘要
The luteinizing hormone/choriogonadotropin receptor (LHR) plays a pivotal
role in reproductive physiology. In the female, its expression in the
ovary is necessary both for ovulation as well as for the initial
maintenance of pregnancy. During follicular development, ovulation,
luteinization, and luteolysis the levels of LHR vary greatly and are often
under complex hormonal control. The cloning of the cDNA for the LHR has
allowed our lab as well as other s to examine the levels of LHR mRNA which
accompany known changes in LHR numbers in the ovary. t has been shown
that there is in fact a very tight correlation between receptor numbers and
mRNA levels, suggesting that regulation of transcriptional and/or post-
transcriptional events related to the LHR gene are critical in the
modulation of LHR receptor numbers. More recently, we have been able to
demonstrate that the FSH(cAMP)-mediated increase in LHR mRNA in
differentiating rat granulosa cells is mediated at least in part by an
increase in the transcription of the LHR gene. Preliminary experiments
also indicate that one or more regions within the 5'flanking region of the
LHR gene mediate this stimulatory effect of cAMP. The experiments
proposed herein are designed to further address the molecular mechanisms
underlying the regulation of the LHR in the ovary, using the rat as a model
system.
Specifically, we propose to:
1. Determine the cis- and trans-acting factors which are involved in the
ovarian expression of the LHR gene.
2. Determine whether FSH(cAMP)) stabilizes LHR mRNA in granulosa cells.
3. Determine the relative changes in LHR gene transcription and LHR mRNA
are known to occur.
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批准号:9223342
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资助金额:$22.88万
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资助金额:$23.99万
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财政年份:2005
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依托单位:
NATIONAL RESEARCH SERVICE AWARD
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批准号:6516910
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资助金额:$14.75万
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财政年份:1998
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG Receptor Regulation in the Ovary
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批准号:6370206
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项目类别:
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资助金额:$23.15万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG RECEPTOR REGULATION IN THE OVARY
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批准号:2207491
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项目类别:
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资助金额:$13.02万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG Receptor Regulation in the Ovary
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批准号:6636920
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项目类别:
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资助金额:$23.15万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG RECEPTOR REGULATION IN THE OVARY
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批准号:2378569
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项目类别:
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资助金额:$16.42万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG Receptor Regulation in the Ovary
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批准号:6520987
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项目类别:
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资助金额:$23.15万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
LH/CG RECEPTOR REGULATION IN THE OVARY
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批准号:2883150
-
项目类别:
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资助金额:$18.04万
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财政年份:1996
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负责人:DEBORAH SEGALOFF
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依托单位:
GONADOTROPIN RECEPTORS
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批准号:3073518
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项目类别:
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资助金额:$6.64万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
GONADOTROPIN RECEPTORS
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批准号:3073519
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项目类别:
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资助金额:$6.75万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
FSH RECEPTOR--STRUCTURE AND FUNCTION
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批准号:2201468
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项目类别:
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资助金额:$12.15万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
-
依托单位:
GONADOTROPIN RECEPTORS
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批准号:2194438
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项目类别:
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资助金额:$6.75万
-
财政年份:1992
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负责人:DEBORAH SEGALOFF
-
依托单位:
GONADOTROPIN RECEPTORS
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批准号:2194437
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项目类别:
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资助金额:$6.64万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
FSH RECEPTOR--STRUCTURE AND FUNCTION
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批准号:3330485
-
项目类别:
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资助金额:$11.62万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
GONADOTROPIN RECEPTORS
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批准号:2194436
-
项目类别:
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资助金额:$6.7万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
FSH RECEPTOR--STRUCTURE AND FUNCTION
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批准号:3330484
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项目类别:
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资助金额:$11.02万
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财政年份:1992
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负责人:DEBORAH SEGALOFF
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依托单位:
海外基金