LH/CG RECEPTOR REGULATION IN THE OVARY

卵巢中 LH/CG 受体的调节

• 批准号: 2378569
• 负责人: DEBORAH SEGALOFF
• 金额: $ 16.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2378569
• 关键词: DNA footprinting; chorionic gonadotropin; female; follicle stimulating hormone; gel mobility shift assay; genetic regulatory element; granulosa cell; hormone receptor; hormone regulation /control mechanism; laboratory rat; luteinizing hormone; nuclear runoff assay; ovary; receptor expression; transcription factor

The luteinizing hormone/choriogonadotropin receptor (LHR) plays a pivotal role in reproductive physiology. In the female, its expression in the ovary is necessary both for ovulation as well as for the initial maintenance of pregnancy. During follicular development, ovulation, luteinization, and luteolysis the levels of LHR vary greatly and are often under complex hormonal control. The cloning of the cDNA for the LHR has allowed our lab as well as other s to examine the levels of LHR mRNA which accompany known changes in LHR numbers in the ovary. t has been shown that there is in fact a very tight correlation between receptor numbers and mRNA levels, suggesting that regulation of transcriptional and/or post- transcriptional events related to the LHR gene are critical in the modulation of LHR receptor numbers. More recently, we have been able to demonstrate that the FSH(cAMP)-mediated increase in LHR mRNA in differentiating rat granulosa cells is mediated at least in part by an increase in the transcription of the LHR gene. Preliminary experiments also indicate that one or more regions within the 5'flanking region of the LHR gene mediate this stimulatory effect of cAMP. The experiments proposed herein are designed to further address the molecular mechanisms underlying the regulation of the LHR in the ovary, using the rat as a model system. Specifically, we propose to: 1. Determine the cis- and trans-acting factors which are involved in the ovarian expression of the LHR gene. 2. Determine whether FSH(cAMP)) stabilizes LHR mRNA in granulosa cells. 3. Determine the relative changes in LHR gene transcription and LHR mRNA are known to occur.

促黄体生成素/绒毛膜促性腺激素受体（LHR）在卵巢癌的发生发展中起着关键作用。 在生殖生理学中的作用 在女性中，它的表现在 卵巢是必要的，既为排卵，以及为最初的 维持怀孕。 在卵泡发育，排卵， 黄体化和黄体溶解时，LHR的水平变化很大， 在复杂的荷尔蒙控制下 LHR cDNA的克隆， 使我们的实验室和其他实验室能够检测LHR mRNA的水平， 伴随卵巢中LHR数量的已知变化。 t已显示 事实上，受体数量和 mRNA水平，这表明转录和/或后- 与LHR基因相关的转录事件在 LHR受体数量的调节。 最近，我们已经能够 表明FSH（cAMP）介导的LHR mRNA增加， 大鼠颗粒细胞的分化至少部分由 增加LHR基因的转录。 初步实验 还表明，所述多肽的5 '侧翼区域内的一个或多个区域 LHR基因介导cAMP的这种刺激作用。 实验 本文提出的是为了进一步解决分子机制， 以大鼠为模型，探讨卵巢中LHR的调节机制 系统 具体而言，我们建议： 1. 确定参与细胞凋亡的顺式和反式作用因子。 LHR基因的卵巢表达。 2. 确定FSH（cAMP）是否稳定颗粒细胞中的LHR mRNA。 3. 确定LHR基因转录和LHR mRNA的相对变化 是已知的。