HIGH THROUGHPUT, LOW COST, SINGLE MOLECULE DNA SEQUENCIN

高通量、低成本、单分子 DNA 测序

• 批准号: 2680263
• 负责人: JAY K TRAUTMAN
• 金额: $ 115.17万
• 依托单位: PRAELUX, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-20 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2680263
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; chemical cleavage; enzyme activity; exonuclease; method development; nucleic acid sequence; nucleotides; photochemistry

DESCRIPTION: SEQ is developing single-molecule detection, identification, manipulation and chemistry capabilities for the purpose of creating a high-speed, low cost DNA sequencer. This sequencing method consists of the isolation and processive exonucleolytic digestion of individual, long (50 kb) strands of DNA. The cleaved nucleotides are immobilized on a surface, in order, and subsequently detected and identified. Importantly, and in contrast to other sequencing methods, SEQ's approach uses native DNA. The present application seeks funding for those aspects of the scheme that require additional research and invention prior to a proof-of-principle demonstration; these are: 1) nucleotide photophysics and photochemistry, 2) nucleotide-surface binding and its effect on nucleotide photophysics and chemistry, and 3) exonuclease processivity and its cleavage-time distribution. When integrated with the advanced engineering portions of the sequencing scheme, the capabilities represented by the accomplishment of the individual specific aims of this application will enable a native-DNA single molecule sequencer. At that time, resolution of the majority of the present uncertainties regarding the efficiency and economy of such a sequencer will be answerable.

描述：SEQ正在开发单分子检测，识别， 操纵和化学能力是为了创建一个 高速，低成本的DNA测序仪。 这种测序方法由 孤立和遗传性外核解体消化，长长（50 KB）DNA链。 切割的核苷酸被固定在表面上， 按顺序进行，随后被检测和识别。 重要的是，在 与其他测序方法形成对比，SEQ的方法使用天然DNA。 这 目前的申请寻求资助该计划的那些方面 在原理证明之前需要其他研究和发明 示范;这些是：1）核苷酸的光体物理和光化学，2） 核苷酸 - 表面结合及其对核苷酸辐射物质的影响和 化学和3）核酸外切酶的加工性及其裂解时间 分配。 与高级工程部分集成时 测序方案，由成就的能力代表 该应用程序的个别特定目标将使本地-DNA单一启用 分子序列。 当时，当前大多数的解决方案 关于此类音序器的效率和经济的不确定性将会 要回答。