HIGH THROUGHPUT, LOW COST, SINGLE MOLECULE DNA SEQUENCIN

描述：SEQ正在开发单分子检测、鉴定、 操作和化学能力，目的是创建 高速、低成本的DNA测序仪。此排序方法由 个体Long(50)的分离和进行性外切消化 Kb)DNA链。裂解的核苷酸被固定在表面上， 并随后被检测和识别。重要的是，在 与其他测序方法相比，SEQ的方法使用的是天然DNA。这个 本申请寻求为该计划的以下方面提供资金 在原则证明之前需要额外的研究和发明 演示：1)核苷酸光物理和光化学，2) 核苷酸-表面结合及其对核苷酸光物理和光物理的影响 化学；3)核酸外切酶的加工性及其切割时间 分发。当与高级工程部分集成时 排序方案，由完成 此应用程序的个别特定目标将实现原生DNA单 分子测序仪。当时，解决目前的大部分问题 关于这种定序器的效率和经济性的不确定性将 要负起责任。

DESCRIPTION: SEQ is developing single-molecule detection, identification, manipulation and chemistry capabilities for the purpose of creating a high-speed, low cost DNA sequencer. This sequencing method consists of the isolation and processive exonucleolytic digestion of individual, long (50 kb) strands of DNA. The cleaved nucleotides are immobilized on a surface, in order, and subsequently detected and identified. Importantly, and in contrast to other sequencing methods, SEQ's approach uses native DNA. The present application seeks funding for those aspects of the scheme that require additional research and invention prior to a proof-of-principle demonstration; these are: 1) nucleotide photophysics and photochemistry, 2) nucleotide-surface binding and its effect on nucleotide photophysics and chemistry, and 3) exonuclease processivity and its cleavage-time distribution. When integrated with the advanced engineering portions of the sequencing scheme, the capabilities represented by the accomplishment of the individual specific aims of this application will enable a native-DNA single molecule sequencer. At that time, resolution of the majority of the present uncertainties regarding the efficiency and economy of such a sequencer will be answerable.