PRESYNAPTIC GLIAL ENHANCEMENT OF SYNAPTIC EFFICACY

人脑中的突触与神经胶质细胞密切相关。 然而，神经胶质细胞在突触的发育和维持中的作用已经 没有得到彻底的调查。来自巴雷斯博士的初步数据 实验室表明，突触在体外没有胶质细胞的情况下形成 疗效不佳。神经胶质细胞可强烈增强脑出血的疗效。 这些突触。我们建议进一步描述神经胶质细胞是如何 增强突触效能。特别是，我们将检验这一假设 神经胶质细胞通过增加突触的可能性来增强突触的效力 突触前递质释放。我们将研究神经胶质细胞如何 调节突触效能。A)神经胶质细胞调节递质吗 合成？B)神经胶质细胞调节递质释放吗？C)是 突触的神经胶质增强所需的电活动 效果如何？D)神经胶质细胞是否增强突触前钙电流？E)做 胶质细胞上调一种或多种突触前蛋白的表达。

Synapses throughout the human brain are closely associated with glia. Yet, the role of glia in the development and maintenance of synapses has not been thoroughly investigated. Preliminary data from Dr. Barres' laboratory indicate that synapses formed in the absence of glia in vitro have low efficacy. Glial cells can strongly enhance the efficacy of these synapses. We propose to further characterize how glial cells enhance synaptic efficacy. In particular, we will test the hypothesis that glia enhance synaptic efficacy by increasing the probability of presynaptic transmitter release. We will investigate how glial cells regulate synaptic efficacy. a) Do glial cells regulate transmitter synthesis? b) Do glial cells regulate transmitter release? c) Is electrical activity required for the glial enhancement of synaptic efficacy? d) Do glial cells enhance presynaptic calcium currents? e) Do glial cells upregulate expression of one or more presynaptic proteins.