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Investigating Astrocyte Diversity and Function in Midbrain Dopaminergic Circuits

研究中脑多巴胺能回路中星形胶质细胞的多样性和功能

基本信息

批准号：
8600322
负责人：
Erik M Ullian
金额：
$ 27.55万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-12-20 至 2015-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8600322
关键词：
Ablation Affect Astrocytes Atlases Attention deficit hyperactivity disorder Axon Biology Brain Brain Stem Brain region Candidate Disease Gene Cell Nucleus Cells Cues Data Data Set Databases Development Disease Dorsal Erinaceidae Fibrous Astrocyte Functional disorder Genes Glues Human In Situ Label Lead Measures Mediating Mental Health Mental disorders Messenger RNA Methods MicroRNAs Midbrain structure Molecular Molecular Profiling Mus Nerve Neurobiology Parkinson Disease Pattern Population Regulation Role Schizophrenia Semaphorin-3A Specific qualifier value Substantia nigra structure Testing Text To specify Transgenic Mice Transgenic Organisms Ventral Tegmental Area Western Blotting abstracting axon guidance clinically relevant dopaminergic neuron embryonic stem cell hindbrain in vivo insight nervous system disorder neural circuit novel tool transcription factor

项目摘要

Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. ABSTRACT Recent studies indicate that astrocytes are spatially diversified, which may have consequence for psychiatric diseases with regional brain dysfunction. However, establishing diversified functions of astrocytes in brain development and disease has been limited by a lack of tools for lineage-specific astrocyte manipulation. Recently, we developed novel transgenic methods to label, purify and characterize regional astrocyte populations in mouse brain. The midbrain and brainstem contain dopaminergic nuclei of the ventral tegmental area (VTA) and substantia nigra (SN) that are implicated in schizophrenia, ADHD and Parkinson's disease. We will test the hypothesis that regionally-specified astrocytes from the ventral midbrain are uniquely suited to support survival and connectivity of dopaminergic projections from the VTA and SN. We will determine molecular diversity of region-restricted midbrain astrocytes as a first step towards uncovering the role of regionally diverse astrocytes in neurological disorders. Deliverables include validated transgenic mouse tools for conditional temporal- spatial labeling, purification of astrocytes throughout the CNS as well as markers of regionally heterogeneous astrocytes subtypes of mid/hindbrain compiled into a public database. The project is intended to reveal signatures of astrocyte subtypes and provide insight into the role of astrocytes in mental health.

在这里输入文本，即应用程序的新抽象信息。这部分必须是no