SOLID STATE STRUCTURAL NMR OF LH1 PROTEIN

在过去的十年里， 结构生物学 一类蛋白质，特别重要， 生物能量转换，膜蛋白，已经开始产生 通过高分辨率方法获得结构信息。这些发展 尽管如此，我们目前对膜蛋白的知识基础 结构是非常有限的; 103球状蛋白质结构已被 报道，但只有101膜蛋白结构是已知的。 此外，大约30%的结构基因 在自由生活的微生物中被认为编码膜蛋白， 一个结论是，膜蛋白结构是一个重要的领域， 被忽视的结构生物学 同样，详细 检查折叠和稳定膜的分子力 蛋白质落后于球状蛋白质。 申请人建议 用固态核磁共振来解决这些问题 谱 高分辨率结构和动力学的一个 寡聚膜蛋白，LH 1捕光复合物， 光合细菌，将被研究。

The last decade has witnessed a remarkable growth of knowledge in structural biology. A class of protein particularly important for biological energy transduction, membrane proteins, has begun to yield structural information by high-resolution methods. These developments notwithstanding, our current knowledge-base of membrane-protein structure is very limited; 103 globular protein structures have been reported, but only 101 membrane protein structures are known. Furthermore, consider that approximately 30 percent of structural genes in a free-living microbe are believed to encode membrane proteins, and one concludes that membrane protein structure is an important area of structural biology that has been overlooked. Likewise, detailed examination of the molecular forces that fold and stabilize membrane proteins has lagged behind globular proteins. The Applicant proposes to undertake novel approaches to these problems with solid state NMR spectroscopy. The high resolution structure and dynamics of an oligomeric membrane protein, the LH1 light-harvesting complex from photosynthetic bacteria, will be studied.