SOLID STATE STRUCTURAL NMR OF LH1 PROTEIN

过去的十年见证了知识的显著增长 结构生物学。一类特别重要的蛋白质，对 生物能量转导，膜蛋白，已经开始产生 通过高分辨率方法获取结构信息。这些事态发展 尽管如此，我们目前的膜蛋白知识库 结构非常有限；103个球状蛋白质结构已经被 但目前已知的膜蛋白结构只有101种。 此外，考虑到大约30%的结构基因 在一种自由生活的微生物中，被认为编码膜蛋白，并且 一个结论是膜蛋白结构是一个重要的领域 被忽视的结构生物学。同样，详细的 对折叠和稳定膜的分子作用力的检验 蛋白质已经落后于球状蛋白质。申请人提出建议 用固体核磁共振对这些问题采取新的方法 光谱学。An的高分辨率结构和动力学 低聚膜蛋白，LH1捕光复合体 光合作用细菌，将被研究。

The last decade has witnessed a remarkable growth of knowledge in structural biology. A class of protein particularly important for biological energy transduction, membrane proteins, has begun to yield structural information by high-resolution methods. These developments notwithstanding, our current knowledge-base of membrane-protein structure is very limited; 103 globular protein structures have been reported, but only 101 membrane protein structures are known. Furthermore, consider that approximately 30 percent of structural genes in a free-living microbe are believed to encode membrane proteins, and one concludes that membrane protein structure is an important area of structural biology that has been overlooked. Likewise, detailed examination of the molecular forces that fold and stabilize membrane proteins has lagged behind globular proteins. The Applicant proposes to undertake novel approaches to these problems with solid state NMR spectroscopy. The high resolution structure and dynamics of an oligomeric membrane protein, the LH1 light-harvesting complex from photosynthetic bacteria, will be studied.