MOLECULAR BIOLOGY OF 11CIS RETNYL ESTER HYDROLASE

目前视觉通路的概念涉及11顺式结构的形成。 异位水解酶从全反式视黄酸酯中提取视黄醇。两人都是11- 顺式视黄酸酯和11-顺式视黄酸酯是由11-顺式视黄酸酯衍生而来。 视黄醇和视觉通路的这个分支点是已知的。虽然 11顺式视网膜作为视觉发色团的命运一直很好 研究了11-顺式视黄酸酯是如何用来供应视网膜的 色素再生的生色团尚不清楚。以前的研究 在我们实验室进行的实验证实了11-Cis的存在 牛视网膜色素中视黄酸酯水解酶活性的研究 上皮(RPE)微体。我们目前的研究主要集中在 11-顺式维甲酸的分离纯化及生化性质 酯水解酶。 在这项教师发展计划中，我们建议扩大 我们目前的生物化学研究涵盖了分子生物学。这个 申请者将连续五个暑期全职工作 约翰·李博士在华盛顿大学生物化学系的实验室 位于圣安东尼奥的德克萨斯大学健康科学中心将克隆和 对编码这种蛋白质的基因进行测序。基因的表达 这种酶的编码也将在牛视网膜色素中进行研究 上皮原代细胞培养。 这项研究之所以重要，是因为对 视觉通路的这一分支是缺乏的。此外，这位教职员工 发展计划还将扩大申请者目前的教学 研究重点从生物化学转向分子生物学。这是 支持他的部门在蜂窝和通信领域的新重点 分子神经生物学。申请者教学的这种扩展和 研究项目还将带来现代生物学概念和技术 致德克萨斯大学旧金山分校少数族裔和非少数族裔学生 安东尼奥。

Current concept of the visual pathway involves the formation of 11-cis retinol from all-trans retinyl esters by an isomerohydrolase. Both 11- cis retinal and 11-cis retinyl esters are derived from this 11-cis retinol and this branch point of the visual pathway is known. Although the fate of 11-cis retinal to serve as visual chromophore has been well studied, exactly how 11-cis retinyl esters are used to supply retinal chromophores for pigment regeneration is not known. Previous studies conducted in our laboratory established the presence of an 11-cis retinyl ester hydrolase activity in the bovine retinal pigment epithelium (RPE) microsomes. Our current research focused on the purification and biochemical characterization of this 11-cis retinyl ester hydrolase enzyme. In this faculty development project, we propose to expand the focus of our current biochemistry research to cover molecular biology. The applicant will work full-time for five consecutive summers in the laboratory of Dr. John Lee at the Department of Biochemistry at the University of Texas Health Science Center in San Antonio to clone and to sequence the gene encoding this protein. Expression of the gene encoding this enzyme will also be studied in bovine retinal pigment epithelial primary cell cultures. This research is important because an understanding of the details of this branch of the visual pathway is lacking. In addition, this faculty development program will also expand the applicant's current teaching and research focus from biochemistry to molecular biology. This is supportive of his department's new focus in the area of cellular and molecular neurobiology. This expansion of the applicant's teaching and research programs will also bring modern biology concepts and techniques to minority and non-minority students at The University of Texas at San Antonio.