DEVELOPMENT OF CAROTID BODY CHEMOTRANSDUCTION

描述：这个为期5年的计划的目的是调查 动脉化学感受的分子机制 后天发育。特别是，假设最终结果是 动脉化学感受器的神经传递是抑制的， 这样，产生的通风不稳定就会受到检测。它更远了 他提出，缺乏对即刻早期基因的诱导 化学感受器神经通路的细胞(即颈动脉小体， 岩神经节、NTS和舌下神经节)将通过兴奋刺激起作用 作为这种抑制的标志。申请者将在现场使用 杂交组织化学：a)以确定 颈动脉小体及其受体细胞中的神经递质及其受体 岩神经节；b)确定发育对 即刻早期基因在化学感受器细胞中的诱导 神经通路；以及c)确定急性和慢性 受体激动剂和拮抗剂对基因表达的影响 外周和中枢化学感受器激活(通过低氧)。

DESCRIPTION: It is the purpose of this 5-year program to investigate molecular mechanisms involved in arterial chemoreception during postnatal development. In particular, the hypothesis that the net result of neurotransmission in arterial chemoreceptors is inhibitory and, as such, produces unstable ventilation will be tested. It is further proposed that the lack of the induction of immediate-early genes in cells of the chemoreceptor neural pathway (i.e., carotid bodies, petrosal ganglia, NTS and hypoglossal) by excitatory stimuli will serve as a marker of this inhibition. The applicant will use in situ hybridization histochemistry: a) to characterize the distribution of neurotransmitters and their receptors in cells of the carotid bodies and petrosal ganglia; b) to determine the effect of development on the induction of the immediate-early gene in cells of the chemoreceptor neural pathways; and c) to determine the effects of acute and chronic receptor agonists and antagonists on gene expression in response to peripheral and central chemoreceptor activation (via hypoxia).