DETERMINANTS OF EPSTEIN BARR VIRUS MUCOSAL PATHOGENESIS

爱泼斯坦巴尔病毒粘膜发病的决定因素

• 批准号: 2749379
• 负责人: JOHN W SIXBEY
• 金额: $ 20.49万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2749379
• 关键词: B lymphocyte; Burkitt's lymphoma; Epstein Barr virus; HIV infections; MDCK cell; antibody receptor; cellular pathology; cellular polarity; clinical research; enzyme linked immunosorbent assay; human subject; immunoglobulin A; interleukin 10; intermolecular interaction; laboratory mouse; mononucleosis; oral leukoplakia; oral mucosa; pathologic process; polymerase chain reaction; transfection /expression vector; virion; virus DNA; virus infection mechanism; virus receptors

DESCRIPTION: The applicant proposes to determine the role of epithelial cell polarity in infection by EBV. Little is known about the early, molecular or otherwise, events in EBV infection, when a transient epithelial phase of infection is presumed to occur or what favors the appearance of epithelial diseases such as OHL, NPL or Burkitt's lymphoma associated with EBV. In preliminary results the applicant provides data to suggest that epithelial cell polarity may play a role, whether the uptake of EBV is mediated by the EBV receptor CR2 or by IgA modulation of infection. To address this issue the applicant has developed a MDCK cell line transformed with either (i) CDNA encoding the rabbit polymeric immunoglobulin receptor (pIgR) or (ii) CD21 (CR2) CDNA expression vector (PR4 CR2). These matched MDCK cell transformants will allow the investigators to follow the fate of EBV strains Bas.8 P3HR-1K and Akata following entry of epithelial cells by each pathway ie CR2 mediated or through IgA modulation. The investigators can examine the transcriptional program and fate of virion DNA as well as cell morphology and physiology via viral infection through both routes of entry. The investigators plan to determine whether EBV induces interleukin-10 (Il-10) in epithelial cells by ELISA, biological essays and eventually RT-PCR in collaboration.

描述：申请人建议确定上皮细胞的作用 EB病毒感染中的细胞极性。关于早期的，我们知之甚少 EBV感染中的分子或其他事件，当一过性上皮细胞 感染的阶段被推定为发生，或者什么有利于出现 与OHL、NPL或Burkitt淋巴瘤相关的上皮性疾病 EB病毒。在初步结果中，申请人提供的数据表明 上皮细胞的极性可能起到一定作用，无论EBV的摄取是 由EBV受体CR2或IgA介导的感染调节。至 针对这一问题，申请人开发了一种MDCK细胞系转化 与(I)编码兔多聚免疫球蛋白受体的CDNA (2)CD21(CR2)CDNA表达载体(PR4CR2)。这些相匹配的 MDCK细胞转化体将使调查人员能够跟踪 EB病毒Bas.8株P3HR-1K和AKATA株进入上皮细胞后 每一条途径都是由CR2介导或通过IgA调节。调查人员 可以检查病毒DNA的转录程序和命运以及 通过两种途径通过病毒感染的细胞形态和生理 进入。 研究人员计划确定EBV是否会诱导白细胞介素10 (IL-10)在上皮细胞中的表达 合作中的RT-PCR。