DETERMINANTS OF EPSTEIN BARR VIRUS MUCOSAL PATHOGENESIS

爱泼斯坦巴尔病毒粘膜发病的决定因素

• 批准号: 6176107
• 负责人: JOHN W SIXBEY
• 金额: $ 21.98万
• 依托单位: LOUISIANA STATE UNIV HSC SHREVEPORT
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176107
• 关键词: B lymphocyte; Burkitt's lymphoma; Epstein Barr virus; HIV infections; MDCK cell; antibody receptor; cellular pathology; cellular polarity; clinical research; enzyme linked immunosorbent assay; human subject; immunoglobulin A; interleukin 10; intermolecular interaction; laboratory mouse; mononucleosis; oral leukoplakia; oral mucosa; pathologic process; polymerase chain reaction; transfection /expression vector; virion; virus DNA; virus infection mechanism; virus receptors

DESCRIPTION: The applicant proposes to determine the role of epithelial cell polarity in infection by EBV. Little is known about the early, molecular or otherwise, events in EBV infection, when a transient epithelial phase of infection is presumed to occur or what favors the appearance of epithelial diseases such as OHL, NPL or Burkitt's lymphoma associated with EBV. In preliminary results the applicant provides data to suggest that epithelial cell polarity may play a role, whether the uptake of EBV is mediated by the EBV receptor CR2 or by IgA modulation of infection. To address this issue the applicant has developed a MDCK cell line transformed with either (i) CDNA encoding the rabbit polymeric immunoglobulin receptor (pIgR) or (ii) CD21 (CR2) CDNA expression vector (PR4 CR2). These matched MDCK cell transformants will allow the investigators to follow the fate of EBV strains Bas.8 P3HR-1K and Akata following entry of epithelial cells by each pathway ie CR2 mediated or through IgA modulation. The investigators can examine the transcriptional program and fate of virion DNA as well as cell morphology and physiology via viral infection through both routes of entry. The investigators plan to determine whether EBV induces interleukin-10 (Il-10) in epithelial cells by ELISA, biological essays and eventually RT-PCR in collaboration.

描述：申请人建议确定上皮细胞的作用， EBV感染的细胞极性。 关于早期的， 分子或其他方面，EBV感染事件，当短暂的上皮细胞 感染阶段被假定发生或什么有利于外观 上皮疾病，如OHL、NPL或伯基特淋巴瘤 EB病毒 在初步结果中，申请人提供的数据表明， 上皮细胞极性可能起作用，无论EBV的摄取是否 由EBV受体CR2介导或由伊加调节感染。 到 为了解决这个问题，申请人已经开发了转化的MDCK细胞系， （i）编码兔多聚免疫球蛋白受体的cDNA （ii）CD 21（CR2）cDNA表达载体（PR 4 CR2）。 这些匹配 MDCK细胞转化体将使研究人员能够跟踪 EBV毒株Bas.8 P3 HR-1 K和Akata在通过免疫细胞化学法进入上皮细胞后， 每个途径即CR2介导的或通过伊加调节。 调查人员 可以检查病毒体DNA的转录程序和命运， 细胞形态学和生理学通过病毒感染通过两种途径 入境 研究人员计划确定EBV是否诱导白细胞介素-10 （IL-10）在上皮细胞中的ELISA，生物学论文，并最终 RT-PCR合作

项目成果

Molecular evidence of ocular Epstein-Barr virus infection.

眼部 Epstein-Barr 病毒感染的分子证据。

• DOI: 10.1086/313932
• 发表时间: 2000
• 期刊: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
• 作者: Slobod,KS;Sandlund,JT;Spiegel,PH;Haik,B;Hurwitz,JL;Conley,ME;Bowman,LC;Benaim,E;Jenkins,JJ;Stocks,RM;Gan,Y;Sixbey,JW
• 通讯作者: Sixbey,JW