LOADING EFFECTS ON THE TEMPOROMANDIBULAR JOINT DISC

颞下颌关节盘的负载效应

• 批准号: 2668253
• 负责人: SUSAN W HERRING
• 金额: $ 5.73万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2668253
• 关键词: SDS polyacrylamide gel electrophoresis; biomechanics; body fluid viscosity; chondroitin sulfates; collagen; disease /disorder model; elasticity; electromyography; fluoroscopy; glycoprotein biosynthesis; implant; jaw movement; mandibular condyle; miniature swine; model design /development; mucopolysaccharides; muscle function; protein structure function; proteoglycan; radiotracer; scanning electron microscopy; temporomandibular joint; tensile strength

The intra-articular disc of the temporomandibular joint (TMJ) serves to improve joint fit, facilitate movements, distribute joint loads, protect articular surfaces, and spread lubrication. Yet relatively little is known about its function in vivo and about the relationship between its chemical structure and mechanical behavior. By examining the properties and characteristics of the disc in normal and nonsurgically altered conditions, this study will address these issues. The aims are (1) to examine the in vivo deformations of the disc during function by implanting a strain transducer into the lateral aspect of the disc, (2) to assess the material properties of the disc by in vitro uniaxial tension tests, (3) to investigate sulfated GAG type, concentration, and synthesis in the disc by electrophoresis gel, liquid scintillation counting of [35S] labeling, and DMB analysis, and (4) to inspect the collagen fiber orientation of the disc. Experimental subjects, miniature pigs, will randomly be placed in groups to function as controls or to wear occlusion altering splints for 2 months. Because the more anterior location of condylar loads should cause disc remodeling, the TMJ discs of animals subjected to anterior displacements of the mandible are expected to show (1) a decrease in the anteroposterior deformation of the intermediate band during function; (2) a diminution of viscoelastic behavior, a higher elastic modulus and a lower ultimate strength point; (3) an increase in GAGs and a size shift in biglycan in the anterior bands and a decrease in proteoglycan synthesis in the intermediate band; and (4) a multidirectional collagen fiber pattern in the intermediate band and a more parallel fiber arrangement in the anterior band. In the long term, the results of this study will provide information needed to formulate computer models of disc behavior and will shed light on the role of the disc in temporomandibular disorders.

颞下颌关节（TMJ）的关节内盘用于 改善关节配合，促进运动，分散关节负荷，保护 关节面，并涂抹润滑。但相对来说， 了解其在体内的功能及其与 化学结构和机械性能。通过检查 正常和非手术改变的椎间盘的特征 在这种情况下，这项研究将解决这些问题。目标是（1） 检查椎间盘在功能期间的体内变形， 将应变传感器植入椎间盘的侧面，（2） 为了通过体外单轴测量评估椎间盘的材料特性， 拉伸试验，（3）研究硫酸化GAG类型、浓度，以及 通过凝胶电泳、液体闪烁在圆盘中合成 [35 S]标记计数和DMB分析，以及（4）检查 椎间盘的胶原纤维取向。实验对象，微型 猪，将随机分组作为对照或 戴咬合改变夹板2个月。因为越靠前 髁突载荷的位置应引起椎间盘重塑，TMJ椎间盘 动物的下颌骨前移位是 预期显示（1）前后变形减少， 功能期间的中间带;（2）粘弹性减少 性能，较高的弹性模量和较低的极限强度点; (3)前壁中GAG的增加和双糖链的大小变化 条带和中间条带中蛋白聚糖合成的减少; 和（4）中间层中的多向胶原纤维图案 前带纤维排列更平行。在 从长远来看，这项研究的结果将提供所需的信息， 制定光盘行为的计算机模型，并将阐明 颞下颌关节紊乱病中椎间盘的作用。