ION CHANNELS IN PANCREATIC CELLS

胰腺细胞中的离子通道

• 批准号: 2608408
• 负责人: STANLEY MISLER
• 金额: $ 15.47万
• 依托单位: BARNES-JEWISH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2608408
• 关键词: calcium channel; calcium flux; dogs; evoked potentials; laboratory rat; pancreatic islet function; pancreatic islets; secretion; tissue /cell culture; voltage /patch clamp

DESCRIPTION: This application explores stimulation-secretion coupling in the beta islet cells of the pancreas. The investigator has hypothesized that a subpopulation of insulin granules are 'loosely co-localized' with Ca2+ channels in the plasmalemma, and these granules are released rapidly in response to local increases in Ca2+ concentration. The specific aims test four predictions of this co-localization model: 1) release should have a distinctive relationship to total Ca2+ entry that should be independent of the conditions initiating that entry; 2) widely-separated depolarizations should evoke insulin release with distinct latency periods; 3) sustained Ca2+ entry should deplete the co-localized pool of granules, resulting in a period of quiescence while that pool is replenished; and 4) conditions that increase the size of the co-localized pool should permit prolonged bouts of secretion. To evaluate these predictions, the investigator will use instrumentation capable of measuring these parameters of stimulation and secretion simultaneously in single beta cells.

描述：该应用程序探讨了刺激-分泌偶联， 胰腺的β胰岛细胞 调查人员假设 胰岛素颗粒的亚群与 质膜上的Ca 2+通道，这些颗粒在细胞内迅速释放， 对局部Ca 2+浓度增加的反应。 具体目标测试 这个共同定位模型的四个预测：1）发布应该有一个 与总Ca 2+进入的独特关系，应独立于 启动进入的条件; 2）广泛分离的去极化 应引起胰岛素释放，具有不同的潜伏期; 3）持续 Ca 2+进入应该耗尽颗粒的共定位池，导致 当该池被补充时的静止期;以及4）条件， 增加共同定位池的大小应该允许长时间的 分泌物 为了评估这些预测，研究人员将使用 能够测量这些刺激参数的仪器， 在单个β细胞中同时分泌。