ION CHANNELS IN PANCREATIC CELLS

胰腺细胞中的离子通道

• 批准号: 6124840
• 负责人: STANLEY MISLER
• 金额: $ 16.41万
• 依托单位: BARNES-JEWISH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6124840
• 关键词: calcium channel; calcium flux; dogs; evoked potentials; laboratory rat; pancreatic islet function; pancreatic islets; secretion; tissue /cell culture; voltage /patch clamp

DESCRIPTION: This application explores stimulation-secretion coupling in the beta islet cells of the pancreas. The investigator has hypothesized that a subpopulation of insulin granules are 'loosely co-localized' with Ca2+ channels in the plasmalemma, and these granules are released rapidly in response to local increases in Ca2+ concentration. The specific aims test four predictions of this co-localization model: 1) release should have a distinctive relationship to total Ca2+ entry that should be independent of the conditions initiating that entry; 2) widely-separated depolarizations should evoke insulin release with distinct latency periods; 3) sustained Ca2+ entry should deplete the co-localized pool of granules, resulting in a period of quiescence while that pool is replenished; and 4) conditions that increase the size of the co-localized pool should permit prolonged bouts of secretion. To evaluate these predictions, the investigator will use instrumentation capable of measuring these parameters of stimulation and secretion simultaneously in single beta cells.

描述：这个应用程序探索刺激-分泌耦合在 胰腺的胰岛细胞。调查员假设 胰岛素颗粒的一个亚群与 质膜中的Ca~(2+)通道，这些颗粒在 对局部钙离子浓度升高的反应。特定目标测试 对这种共同本地化模式的四个预测：1)版本应该有一个 与总钙离子进入的独特关系应独立于 启动进入的条件；2)广泛分离的去极化 应以不同的潜伏期激发胰岛素释放；3)持续 进入Ca2+应该会耗尽共局域化的颗粒池，导致 在该池被补充时的静止期；以及4)满足以下条件的条件 增加共同本地化池的大小应允许长时间的 分泌物。为了评估这些预测，调查人员将使用 能够测量这些刺激和参数的仪器 单个β细胞同时分泌。

项目成果

Role of voltage-dependent ionic currents in coupling glucose stimulation to insulin secretion in canine pancreatic islet B-cells.

电压依赖性离子电流在犬胰岛 B 细胞中葡萄糖刺激与胰岛素分泌耦合中的作用。

• DOI: 10.1007/bf01994357
• 发表时间: 1991
• 期刊: The Journal of membrane biology
• 作者: Pressel,DM;Misler,S
• 通讯作者: Misler,S

Stimulus-secretion coupling in beta-cells of transplantable human islets of Langerhans. Evidence for a critical role for Ca2+ entry.

可移植的人胰岛β细胞中的刺激-分泌耦合。

• DOI: 10.2337/diab.41.6.662
• 发表时间: 1992
• 期刊: Diabetes
• 影响因子: 7.7
• 作者: Misler,S;Barnett,DW;Pressel,DM;Gillis,KD;Scharp,DW;Falke,LC
• 通讯作者: Falke,LC

Unifying concepts in stimulus-secretion coupling in endocrine cells and some implications for therapeutics.

统一内分泌细胞刺激-分泌耦合的概念以及对治疗的一些影响。

• DOI: 10.1152/advan.90213.2008
• 发表时间: 2009
• 期刊: Advances in physiology education
• 影响因子: 2.1
• 作者: Misler,Stanley

Increased islet apoptosis in Pdx1+/- mice.

• DOI: 10.1172/jci16537
• 发表时间: 2003-04
• 期刊: The Journal of clinical investigation
• 作者: James D. Johnson;Noreen T. Ahmed;D. Luciani;Zhi-qiang Han;Hung D. Tran;J. Fujita;S. Misler;H. Edlund;K. Polonsky

Long-term monitoring of depolarization-induced exocytosis from adrenal medullary chromaffin cells and pancreatic islet B cells using "perforated patch recording".

使用“穿孔贴片记录”长期监测肾上腺髓质嗜铬细胞和胰岛 B 细胞的去极化诱导的胞吐作用。

• DOI: 10.1111/j.1749-6632.1991.tb36528.x
• 发表时间: 1991
• 期刊: Annals of the New York Academy of Sciences
• 影响因子: 5.2
• 作者: Gillis,KD;Pun,RY;Misler,S
• 通讯作者: Misler,S