CALCIUM IONS AND LYMPHOCYTE ACTIVATION

描述：除了调节神经系统的电活动外， 系统，离子通道介导非常不同的细胞机制内的 免疫系统 最早的信号传导抗原结合至 T淋巴细胞和肥大细胞中的表面受体包括 离子通道和胞质[Ca 2 +]i的升高。 “[Ca 2 +]i信号”是 通过离子通道的开放产生，并将膜受体连接到 基因表达、淋巴因子分泌和细胞增殖， 免疫反应。 强调单细胞方法， 研究者们提出了视频成像和膜片钳实验， 阐明了将膜受体与效应器功能联系起来的机制， 如细胞增殖、基因表达、细胞运动性和 免疫调节分子。 对T淋巴细胞的拟议实验是 分为两个部分，对应于以下事件的顺序 抗原刺激：（1）来自抗原结合的细胞内信号传导 膜受体的[Ca 2 +]i信号;（2）激活的 白细胞介素2基因和其他信号通路通过升高[Ca 2 +]i。 一 本课题的目的是应用光学技术研究[Ca 2 +]i 刺激的原代T细胞中的信号传导、运动性和基因表达 生理配体 研究人员将使用报告基因， 动态地可视化T细胞活化，以便将膜和 第二信使机制的效应功能。 他们正在调查 抗原呈递过程中细胞之间的相互作用，以及 将膜受体与基因连接的细胞内信号传导机制 表情

DESCRIPTION: In addition to regulating electrical activity in the nervous system, ion channels mediate very different cellular mechanisms within the immune system. The earliest events signaling the binding of antigen to surface receptors in T lymphocytes and mast cells include the activation of ion channels and a rise in cytosolic [Ca2+]i. The "[Ca2+]i signal" is generated by the opening of ion channels, and links membrane receptors to gene expression, lymphokine secretion and cell proliferation essential to the immune response. With emphasis on a single-cell approach, the investigators are proposing video-imaging and patch-clamp experiments to clarify mechanisms which link membrane receptors to effector functions such as cell proliferation, gene expression, cell motility, and secretion of immunomodulatory molecules. The proposed experiments on T lymphocytes are divided into two sections corresponding to the sequence of events following antigen stimulation: (1) intracellular signaling from the engagement of membrane receptors to the [Ca2+]i signal; (2) activation of the interleukin-2 gene and other signaling pathways by the rise in [Ca2+]i. One goal of this project is to apply optical techniques to investigate [Ca2+]i signaling, motility, and gene expression in primary T-cells stimulated by physiological ligands. The investigators will use reporter genes to visualize T-cell activation dynamically in order to relate membrane and second-messenger mechanisms to effector function. They are investigating interactions between cells during antigen presentation, as well as intracellular signaling mechanisms that link membrane receptors with gene expression.