DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS
DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS
批准号:
6119307
负责人:
MICHAEL D CAHALAN
金额:
$2.46万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-04-01 至 2000-03-31
中文摘要
重点是单细胞方法,我们建议
使用以下组合对抗原特异性T淋巴细胞进行的实验
光学技术-共焦显微镜、双光子显微镜和
光镊-研究膜受体的动力学
抗原呈递过程中的相互作用。 我们的实验室正在使用
生物物理技术来研究信号机制,
用于监测细胞活化的生物化学和光学测定,以及
基因修饰细胞以探测分子机制。 的
T淋巴细胞和抗原呈递细胞的实验
将重点关注来自参与的信号的动态,
膜受体的[Ca2 +] i信号。 这个项目的一个目标是
为了应用光学技术来研究抗原识别,
[Ca2刺激的T细胞中的+] i信号传导、运动性和基因表达
通过生理配体。 我们将使用报告基因来可视化
T细胞动态活化,以将膜和硒
第二信使机制到效应器功能。 我们正在调查
抗原呈递过程中细胞之间的相互作用,以及
细胞内信号传导机制将膜受体与
基因表达。
英文摘要
With emphasis on a single-cell approach, we are proposing
experiments on antigen-specific T lymphocytes using a combination of
optical techniques - confocal microscopy, two-photon microscopy, and
optical tweezers - to investigate the dynamics of membrane receptor
interactions during antigen presentation. Our laboratory is using
biophysical techniques to investigate signaling mechanisms,
biochemical and optical assays to monitor cell activation, and
genetically modified cells to probe molecular mechanisms. The
proposed experiments on T lymphocytes and antigen-presenting cells
will focus on the dynamics of signaling from the engagement of
membrane receptors to the [Ca2+]i signal. One goal of this project is
to apply optical techniques to investigate antigen recognition,
[Ca2+]i signaling, motility, and gene expression in T cells stimulated
by physiological ligands. We will use reporter genes to visualize
T-cell activation dynamically in order to relate membrane and se
cond-messenger mechanisms to effector function. We are investigating
interactions between cells during antigen presentation, as well as
intracellular signaling mechanisms that link membrane receptors with
gene expression.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Molecular Mechanisms of lon Channels in T Lymphocytes
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批准号:10375085
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项目类别:
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资助金额:$4.78万
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财政年份:2021
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负责人:MICHAEL D CAHALAN
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依托单位:
Cellular and Molecular Mechanisms of Regulatory T Cells in EAE
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批准号:9301460
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资助金额:$49.89万
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财政年份:2016
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负责人:MICHAEL D CAHALAN
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依托单位:
A Transgenic Mouse Line to Map Cell-Type Specific Calcium Signals In Vivo
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批准号:9001251
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项目类别:
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资助金额:$23.18万
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财政年份:2015
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负责人:MICHAEL D CAHALAN
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依托单位:
Immunology Research Training Program
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批准号:8486365
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项目类别:
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资助金额:$11.25万
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财政年份:2005
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负责人:MICHAEL D CAHALAN
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依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6336413
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项目类别:
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资助金额:$22.95万
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财政年份:2000
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负责人:MICHAEL D CAHALAN
-
依托单位:
DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS
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批准号:6308190
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项目类别:
-
资助金额:$2.46万
-
财政年份:2000
-
负责人:MICHAEL D CAHALAN
-
依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6203282
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项目类别:
-
资助金额:$22.95万
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财政年份:1999
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负责人:MICHAEL D CAHALAN
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依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6216526
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项目类别:
-
资助金额:$22.95万
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财政年份:1999
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负责人:MICHAEL D CAHALAN
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依托单位:
T CELL ANTIGEN RECOGNIT DYNAMIC PROBED W/ OPTICAL METHOD: CALCIUM & GENE EXPRESS
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批准号:6220406
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项目类别:
-
资助金额:$3.09万
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财政年份:1999
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负责人:MICHAEL D CAHALAN
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依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6296101
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项目类别:
-
资助金额:$22.95万
-
财政年份:1999
-
负责人:MICHAEL D CAHALAN
-
依托单位:
DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS
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批准号:6280328
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项目类别:
-
资助金额:$7.8万
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财政年份:1998
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负责人:MICHAEL D CAHALAN
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依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6102942
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项目类别:
-
资助金额:$22.95万
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财政年份:1998
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负责人:MICHAEL D CAHALAN
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依托单位:
CALCIUM IONS AND LYMPHOCYTE ACTIVATION
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批准号:6018755
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项目类别:
-
资助金额:$29.12万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
CORE--OPTICAL BIOLOGY FACILITY
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批准号:6237436
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项目类别:
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资助金额:$22.58万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
Functional Imaging of Lymphocyte Motility and Cell Interactions in Lymph Node
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批准号:7212230
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项目类别:
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资助金额:$41.45万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
Functional immuno-imaging of lymphocyte motility and cell interactions in lymph n
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批准号:8054736
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项目类别:
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资助金额:$39.24万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
The Role of Calcium Ions in Lymphocyte Activation
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批准号:6624293
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项目类别:
-
资助金额:$31.74万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
CALCIUM IONS AND LYMPHOCYTE ACTIVATION
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批准号:2022281
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项目类别:
-
资助金额:$29.96万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
The Role of Calcium Ions in Lymphocyte Activation
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批准号:6473546
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项目类别:
-
资助金额:$35.42万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
The Role of Calcium Ions in Lymphocyte Activation
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批准号:6876177
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项目类别:
-
资助金额:$37.35万
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财政年份:1997
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负责人:MICHAEL D CAHALAN
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依托单位:
海外基金