DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS

重点是单细胞方法，我们建议 使用以下组合对抗原特异性T淋巴细胞进行的实验 光学技术-共焦显微镜、双光子显微镜和 光镊-研究膜受体的动力学 抗原呈递过程中的相互作用。 我们的实验室正在使用 生物物理技术来研究信号机制， 用于监测细胞活化的生物化学和光学测定，以及 基因修饰细胞以探测分子机制。 的 T淋巴细胞和抗原呈递细胞的实验 将重点关注来自参与的信号的动态， 膜受体的[Ca2 +] i信号。 这个项目的一个目标是 为了应用光学技术来研究抗原识别， [Ca2刺激的T细胞中的+] i信号传导、运动性和基因表达 通过生理配体。 我们将使用报告基因来可视化 T细胞动态活化，以将膜和硒 第二信使机制到效应器功能。 我们正在调查 抗原呈递过程中细胞之间的相互作用，以及 细胞内信号传导机制将膜受体与 基因表达。

With emphasis on a single-cell approach, we are proposing experiments on antigen-specific T lymphocytes using a combination of optical techniques - confocal microscopy, two-photon microscopy, and optical tweezers - to investigate the dynamics of membrane receptor interactions during antigen presentation. Our laboratory is using biophysical techniques to investigate signaling mechanisms, biochemical and optical assays to monitor cell activation, and genetically modified cells to probe molecular mechanisms. The proposed experiments on T lymphocytes and antigen-presenting cells will focus on the dynamics of signaling from the engagement of membrane receptors to the [Ca2+]i signal. One goal of this project is to apply optical techniques to investigate antigen recognition, [Ca2+]i signaling, motility, and gene expression in T cells stimulated by physiological ligands. We will use reporter genes to visualize T-cell activation dynamically in order to relate membrane and se cond-messenger mechanisms to effector function. We are investigating interactions between cells during antigen presentation, as well as intracellular signaling mechanisms that link membrane receptors with gene expression.