DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS

在强调单细胞方法的同时，我们建议 抗原呈递过程中的相互作用。我们的实验室正在使用 生物物理技术来研究信号机制， 生物化学和光学分析，以监测细胞激活，以及 转基因细胞来探索分子机制。这个 将重点介绍来自以下项目的信号动态 这个项目的一个目标是 为了应用光学技术来研究抗原识别， 发挥效应器功能的条件信使机制。我们正在调查 在抗原呈递期间细胞之间的相互作用，以及 膜受体与细胞内信号转导机制 基因表达。

With emphasis on a single-cell approach, we are proposing experiments on antigen-specific T lymphocytes using a combination of optical techniques - confocal microscopy, two-photon microscopy, and optical tweezers - to investigate the dynamics of membrane receptor interactions during antigen presentation. Our laboratory is using biophysical techniques to investigate signaling mechanisms, biochemical and optical assays to monitor cell activation, and genetically modified cells to probe molecular mechanisms. The proposed experiments on T lymphocytes and antigen-presenting cells will focus on the dynamics of signaling from the engagement of membrane receptors to the [Ca2+]i signal. One goal of this project is to apply optical techniques to investigate antigen recognition, [Ca2+]i signaling, motility, and gene expression in T cells stimulated by physiological ligands. We will use reporter genes to visualize T-cell activation dynamically in order to relate membrane and se cond-messenger mechanisms to effector function. We are investigating interactions between cells during antigen presentation, as well as intracellular signaling mechanisms that link membrane receptors with gene expression.