DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS

使用光学方法探测 T 细胞抗原识别的动力学

• 批准号: 6308190
• 负责人: MICHAEL D CAHALAN
• 金额: $ 2.46万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308190
• 关键词: biological products; biomedical resource; human tissue; immunology; inflammation; lasers; minerals; model design /development

With emphasis on a single-cell approach, we are proposing experiments on antigen-specific T lymphocytes using a combination of optical techniques - confocal microscopy, two-photon microscopy, and optical tweezers - to investigate the dynamics of membrane receptor interactions during antigen presentation. Our laboratory is using biophysical techniques to investigate signaling mechanisms, biochemical and optical assays to monitor cell activation, and genetically modified cells to probe molecular mechanisms. The proposed experiments on T lymphocytes and antigen-presenting cells will focus on the dynamics of signaling from the engagement of membrane receptors to the [Ca2+]i signal. One goal of this project is to apply optical techniques to investigate antigen recognition, [Ca2+]i signaling, motility, and gene expression in T cells stimulated by physiological ligands. We will use reporter genes to visualize T-cell activation dynamically in order to relate membrane and se cond-messenger mechanisms to effector function. We are investigating interactions between cells during antigen presentation, as well as intracellular signaling mechanisms that link membrane receptors with gene expression.

重点强调单细胞方法，我们建议 结合使用抗原特异性 T 淋巴细胞的实验 光学技术 - 共焦显微镜、双光子显微镜和 光镊 - 研究膜受体的动力学 抗原呈递过程中的相互作用。 我们实验室正在使用 研究信号传导机制的生物物理技术， 用于监测细胞活化的生化和光学测定，以及 基因改造细胞来探索分子机制。 这 提议的 T 淋巴细胞和抗原呈递细胞实验 将重点关注参与的信号动态 [Ca2+]i 信号的膜受体。 该项目的目标之一是 应用光学技术研究抗原识别， 刺激 T 细胞中的 [Ca2+]i 信号传导、运动性和基因表达 通过生理配体。 我们将使用报告基因来可视化 T 细胞动态激活，以便将膜和细胞联系起来 效应器功能的条件信使机制。 我们正在调查 抗原呈递过程中细胞之间的相互作用，以及 连接膜受体的细胞内信号传导机制 基因表达。