HLA-G AND MATERNAL AND FETAL IMMUNITY

HLA-G 与母体和胎儿免疫力

• 批准号: 2721470
• 负责人: JOAN Sherar HUNT
• 金额: $ 7.5万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2721470
• 关键词: antibody formation; apoptosis; bioassay; cellular immunity; cytokine; diagnosis design /evaluation; enzyme linked immunosorbent assay; histocompatibility antigens; histology; human pregnant subject; immunomodulators; immunosuppression; in situ hybridization; lymphocyte; placenta; pregnancy immunology; prenatal diagnosis; protein isoforms; serology /serodiagnosis

DESCRIPTION (Adapted from applicant's description): The long-term goal of this research is to investigate the following hypothesis: Soluble HLA-G derived from placentas has a profound influence on maternal and fetal immunity that benefits semiallogeneic pregnancy. To explore this postulate experimentally, the investigator plans to develop new tools and key preliminary data. Specific Aims are: 1) To prepare and characterize molecular probes and antibodies for identification of specific HLA-G transcripts and proteins; 2) To: a) develop an in vitro mammalian expression system to produce soluble HLA-G; (b) use the soluble HLA-G to establish an ELISA assay; and c) use the ELISA assay to test sera from nonpregnant and pregnant women for soluble HLA-G; and 3) To determine whether or not the placenta is a source of soluble HLA-G by ascertaining transcript and protein production patterns in specific cell lineages in first trimester and term placentas using in situ hybridization and immunohistology.

描述（根据申请人的描述改编）： 本研究旨在探讨以下假说：可溶性HLA-G 对母体和胎儿有着深远的影响 有利于半同种异体妊娠的免疫力。 为了探索这个假设 实验上，研究人员计划开发新的工具和关键 初步数据。 具体目的是：1）制备和表征 用于鉴定特异性HLA-G的分子探针和抗体 转录物和蛋白质; 2）为了：a）开发体外哺乳动物 表达系统以产生可溶性HLA-G;（B）使用可溶性HLA-G以 建立ELISA测定;和c）使用ELISA测定来测试来自以下的血清： 非孕妇和孕妇可溶性HLA-G;和3）确定 通过确定胎盘是否是可溶性HLA-G的来源， 特定细胞谱系中的转录本和蛋白质生产模式 使用原位杂交技术检测妊娠早期和足月胎盘， 免疫组织学