CLONING IRON RESPONSIVE GENES IN S MUTANS

这是一份修订后的、大大简化的提案，旨在调查 铁在变形链球菌致龋性中的作用。 和以前一样，前提是铁在糖尿病的发病机制中起作用。 龋齿。这一假说是建立在大量文献的基础上的 细菌病原体，在某些情况下，包含复杂的机制 用来获取铁。此外，众所周知，铁 收购与既定的毒力机制直接相关。在……里面 在修改后，笔者将继续论证铁在其中的作用 在龋病病因学中建立以下机制： 变形链球菌获取铁的途径；对铁敏感的鉴定 变形链球菌中的蛋白质以及铁的克隆和鉴定 生物体中的反应基因(S)。未来的实验将继续进行 铁反应基因和大鼠诱变的研究进展 龋齿实验，以证实基因在龋病发生中的作用；以及， 最后通过核糖核酸和基因融合实验来确定铁 反应基因受转录调控。

This is a revised, and greatly streamlined, proposal to investigate the role that iron may play in the cariogenic ability of Streptococcus mutans. The premise, as before, is that irons plays a role in pathogenesis of caries. The hypothesis is based on the large literature for other bacterial pathogens which contains, in some cases, elaborate mechanisms for the acquisition of iron. Furthermore, it is known that iron acquisition is directly tied in with established virulence mechanisms. In the revision, the author will pursue the argument that iron plays a role in the etiology of dental caries by establishing the following: mechanisms by which S. mutans acquires iron; identification of iron-responsive proteins in S. mutans; and the cloning and characterization of iron responsive gene(s) in the organism. Future experiments will hold forth on the previous aims concerning mutagenesis of iron responsive genes and rat caries experiments to verify the role of the genes in cariogenesis; and, finally RNA and gene fusion experiments to determine whether iron responsive genes are transcriptionally regulated.