CYP1A1 AS A BIOMARKER OF EXPOSURE AND SUSCEPTIBILITY

CYP1A1 作为暴露和易感性的生物标志物

• 批准号: 2770794
• 负责人: SEYMOUR J GARTE
• 金额: $ 2.17万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770794
• 关键词: African American; aromatic hydrocarbon receptor; biomarker; cancer risk; carbopolycyclic compound; carcinogens; clinical research; cytochrome P450; environmental toxicology; gene environment interaction; gene expression; genetic polymorphism; hair follicle; human subject; human tissue; lymphocyte; method development; neoplasm /cancer epidemiology; nucleic acid sequence; polymerase chain reaction; smoking; tobacco abuse

DESCRIPTION (Adapted from the Investigator's Abstract): The focus of this project is the use of CYP1A1 gene to monitor human exposure to aromatic hydrocarbons, and to explore the molecular basis of CYP1A1 polymorphisms as cancer susceptibility factors in African Americans. A new quantitative reverse transcriptase PCR method for measurement of gene expression in various indicator tissues (frozen blood, nasal epithelial cells, exfoliated bladder epithelial cells), of people exposed to cigarette smoke, creosote and charcoal broiled foods will be developed and validated. This method, which uses a homologous internal standard (HIS) for accurate quantitation, has the promise of becoming a valuable tool in the development of new biomarkers of exposure based on gene expression in small samples of fresh or stored human tissue. The investigators will extend their research into the mechanisms of individual differences in cancer susceptibility, by investigating the biochemical functional significance of two CYP1A1 restriction polymorphisms that they have found to be linked with cancer risk in African Americans. The investigators will determine whether either of these RFLPs are linked with other polymorphisms in the CP1A1, AHR or ARNT genes in African Americans.

描述(改编自《调查者摘要》)：这篇文章的重点 该项目是使用CYP1A1基因来监测人类接触芳香烃的情况 并探讨细胞色素P4501A1基因多态性的分子基础 非裔美国人中的癌症易感因素。一种新的定量 逆转录聚合酶链式反应技术用于基因表达的检测 各种指示性组织(冰冻血液、鼻上皮细胞、脱落 膀胱上皮细胞)，暴露在香烟烟雾中的人，杂酚油 木炭烧烤食品将被开发和验证。这种方法， 其使用同源内部标准(HIS)进行精确定量， 有希望成为新的开发的宝贵工具 基于基因表达的暴露生物标记物在新鲜或 储存的人体组织。调查人员将把他们的研究扩展到 癌症易感性的个体差异机制，由 两种细胞色素P1A1的生化功能意义探讨 他们发现与癌症风险相关的限制性多态 在非裔美国人身上。调查人员将确定其中一人是否 这些RFLP与CP1A1、AHR或ARNT中的其他多态连锁 非裔美国人的基因。