CYP1A1 AS A BIOMARKER OF EXPOSURE AND SUSCEPTIBILITY

描述（改编自研究者摘要）：本文的重点 项目是利用CYP1A1基因监测人类接触芳香族化合物 碳氢化合物，并探讨CYP1A1多态性的分子基础， 非裔美国人的癌症易感因素。 一种新的定量 逆转录酶PCR法检测基因表达 各种指示组织（冷冻血液、鼻上皮细胞、脱落 膀胱上皮细胞），暴露于香烟烟雾，杂酚油的人 炭烤食品将被开发和验证。 这种方法， 其使用同源内标（HIS）进行精确定量， 有希望成为开发新的 基于小样本新鲜或新鲜的基因表达的暴露生物标志物 储存的人体组织 调查人员将把他们的研究扩展到 癌症易感性个体差异的机制， 研究两个CYP1A1的生化功能意义 他们发现与癌症风险有关的限制性多态性 在非裔美国人中。 调查人员将确定 这些RFLP与CP1A1、AHR或ARNT中的其他多态性连锁 非裔美国人的基因

DESCRIPTION (Adapted from the Investigator's Abstract): The focus of this project is the use of CYP1A1 gene to monitor human exposure to aromatic hydrocarbons, and to explore the molecular basis of CYP1A1 polymorphisms as cancer susceptibility factors in African Americans. A new quantitative reverse transcriptase PCR method for measurement of gene expression in various indicator tissues (frozen blood, nasal epithelial cells, exfoliated bladder epithelial cells), of people exposed to cigarette smoke, creosote and charcoal broiled foods will be developed and validated. This method, which uses a homologous internal standard (HIS) for accurate quantitation, has the promise of becoming a valuable tool in the development of new biomarkers of exposure based on gene expression in small samples of fresh or stored human tissue. The investigators will extend their research into the mechanisms of individual differences in cancer susceptibility, by investigating the biochemical functional significance of two CYP1A1 restriction polymorphisms that they have found to be linked with cancer risk in African Americans. The investigators will determine whether either of these RFLPs are linked with other polymorphisms in the CP1A1, AHR or ARNT genes in African Americans.