EMF AND EARLY BONE DEVELOPMENT

描述：暴露在极低频率(ELF)磁场中 被证明对生物系统有许多重大影响 并对可能的健康危害提出了担忧。 有了这样的曝光率。ELF的全谱生物活性 菲尔兹是未知的，关于这一点也有不同的说法。 一方面，致癌潜力和潜在的治疗益处 在另一端。 这项提案中计划的研究将检验这样的假设 暴露在幅度和曝光量不同的60赫兹磁场中 持续时间可能会导致细胞间关系的变化 小鼠体内发育模型中的增殖与分化 软骨内骨化。这个模型显示了发育顺序。 增殖和分化，因为它一直很好 在形态、生化和分子方面，它的特征是 作为检验ELF场影响的一个很好的测试系统 暴露于基因组对增殖和增殖的调节 差异化。该模式适用于细胞调节的问题 一般来说，它与骨骼有重要的细胞相似之处 胚胎发生和畸形学，生长和修复，通过外推， 致癌作用。 Stein和Lian的观察表明，核扩散- 实验性软骨内存在分化关系 骨化，这种关系可以通过激素和 其他影响，使得差异化可以加速或 发育迟缓，对增殖有互惠的影响。观察到的 Aaron和Ciombor，具有相同的实验性内软骨系统 骨化，表明细胞外基质的精细化 蛋白多糖与蛋白多糖核心蛋白的mRNA表达 可以通过暴露在ELF磁场中加速，并且这种刺激 驻留在祖细胞池中。这两组观察 形成了这一提议的试验性方法的基础。 他们的实验方法将测量相关基因的表达 并将评估ELF字段是否可以用作信令机制和 改变基因组对增殖和分化的调控。 这些结果将有助于深入了解基波的ELF场效应。 具有广泛生物学适用性的调控过程。

DESCRIPTION: Exposure to extremely low frequency (ELF) fields has been shown to have a number of significant effects upon biological systems and concern has been raised about possible health hazards associated with that exposure. The full spectrum of biological activities of ELF fields is not known, and varying claims have been made regarding oncogenic potential on the one hand, and potential therapeutic benefits on the other. The studies planned in this proposal will examine the hypothesis that exposure to 60 Hz magnetic fields of varying amplitude and exposure duration may produce changes in the relationship of cellular proliferation and differentiation in an in vivo developmental model of endochondral ossification. This model exhibits developmental sequences of proliferation and differentiation and, since it has been well characterized in morphological, biochemical and molecular terms, it serves as an excellent test system to examine the effects of ELF field exposure upon the genomic regulation of proliferation and differentiation. The mode is applicable to issues of cell regulation in general and has important cellular analogies to skeletal embryogenesis and teratology, growth and repair and, by extrapolation, to oncogenesis. The observations of Stein and Lian indicate that a proliferation- differentiation relationship exists in experimental endochondral ossification and that this relationship can be modified by hormonal and other influences such that differentiation can be accelerated or retarded with reciprocal effects on proliferation. The observations of Aaron and Ciombor, with the same system of experimental endochondral ossification, indicate that the elaboration of extracellular matrix proteoglycan and the expression of mRNA for proteoglycan core protein can be accelerated by exposure to ELF fields and that this stimulation resides in the progenitor cell pool. These two sets of observations form the basis for the experimental approach for this proposal. Their experimental approach will measure expression of pertinent genes and will assess whether ELF fields can act as a signaling mechanism and alter the genomic regulation of proliferation and differentiation. These results will lend insight into ELF field effects upon fundamental regulatory processes with broad biological applicability.