CYTOPLASMIC MRNA TRANSPORT IN XENOPUS OOCYTES

描述：本提案的目标是了解 控制非洲爪蟾卵母细胞上Vg1 RNA的定位。 先前实验 在Schnapp的实验室中已经表明，这种定位需要存在 存在于所述多肽的3'UTR中的四个不同重复中的每一个的至少一个拷贝， mRNA。 五核苷酸重复UUCAC（E2）似乎起主要作用， 是这项资助的重点 Schnapp已经发现了一种蛋白质，vera， 与VgLE结合，最有可能是通过与VgLE直接物理结合， E2重复。 由于Vera是一种ER相关蛋白，Schnapp提出， 它在将Vg1RNA定位于ER中起作用。 Schnapp还成功地分离出了含有Vg 1的RNP粒子。 定位元件（VgLE）。 所有四种VgLE重复类型都是必需的， VgLE在粒子中的关联。 这与早期的 观察到，只有三个（E1，E2，E4）需要与 蛋白质，这反过来表明，后者是不够的， 粒子组装

DESCRIPTION: The goal of this proposal is to understand the mechanisms that govern localization of Vg1 RNA on the Xenopus oocyte. Previous experiments in Schnapp's lab have shown that this localization requires the presence of at least one copy each of four different repeats present in the 3'UTR of the mRNA. A five nucleotide repeat UUCAC (E2) appears to play a major role and is the focus of much of this grant. Schnapp has identified a protein, vera, that binds to the VgLE, most likely by direct physical association with the E2 repeat. Because Vera is an ER associated protein, Schnapp proposes that it plays a role in localizing Vg1RNA to the ER. Schnapp has also succeeded in isolating a RNP particle containing the Vg1 localization element (VgLE). All four VgLE repeat types are required for the VgLE's association in the particle. This contrasts with the earlier observation that only three (E1,E2,E4) are required for association with vera protein, suggesting in turn that the latter is not sufficient for particle assembly.