CYTOPLASMIC MRNA TRANSPORT IN XENOPUS OOCYTES

爪蟾卵母细胞中的细胞质 mRNA 转运

• 批准号: 6543106
• 负责人: BRUCE Jeffrey SCHNAPP
• 金额: $ 17.23万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6543106
• 关键词: Xenopus oocyte; cytoplasm; endoplasmic reticulum; gel mobility shift assay; messenger RNA; molecular biology; nucleic acid sequence; protein binding; protein localization; protein purification; protein sequence; ribonucleoproteins

DESCRIPTION: The goal of this proposal is to understand the mechanisms that govern localization of Vg1 RNA on the Xenopus oocyte. Previous experiments in Schnapp's lab have shown that this localization requires the presence of at least one copy each of four different repeats present in the 3'UTR of the mRNA. A five nucleotide repeat UUCAC (E2) appears to play a major role and is the focus of much of this grant. Schnapp has identified a protein, vera, that binds to the VgLE, most likely by direct physical association with the E2 repeat. Because Vera is an ER associated protein, Schnapp proposes that it plays a role in localizing Vg1RNA to the ER. Schnapp has also succeeded in isolating a RNP particle containing the Vg1 localization element (VgLE). All four VgLE repeat types are required for the VgLE's association in the particle. This contrasts with the earlier observation that only three (E1,E2,E4) are required for association with vera protein, suggesting in turn that the latter is not sufficient for particle assembly.

描述：本提案的目标是了解以下机制： 控制 Vg1 RNA 在爪蟾卵母细胞上的定位。 之前的实验 Schnapp 的实验室表明，这种本地化需要存在 3'UTR 中存在的四个不同重复序列中各至少有一个副本 mRNA。 五核苷酸重复 UUCAC (E2) 似乎发挥着重要作用 是这笔赠款大部分的重点。 施纳普已经鉴定出一种蛋白质，维拉， 与 VgLE 结合，很可能是通过与 E2 重复。 由于 Vera 是一种 ER 相关蛋白，Schnapp 提出 它在将 Vg1RNA 定位到 ER 中发挥作用。 Schnapp 还成功分离出含有 Vg1 的 RNP 粒子 定位元件（VgLE）。 所有四种 VgLE 重复类型都是必需的 VgLE 在粒子中的关联。 这与之前的对比 观察到只需要三个（E1，E2，E4）与关联 维拉蛋白，反过来表明后者不足以 粒子组装。