Nipkow-disk Confocal Microscope for Live-Cell Imaging

用于活细胞成像的 Nipkow 盘共焦显微镜

• 批准号: 6581981
• 负责人: BRUCE Jeffrey SCHNAPP
• 金额: $ 32.44万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581981
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): This application supports the purchase of a Perkin-Elmer Ultraview LCI confocal microscope. This newly designed microscope, based on a recent enhancement in Nipkow disk technology, represents a major advance in confocal imaging of living cells. Compared with microscopes that use laser scanning, the Ultraview enables far faster image acquisition, real-time confocal viewing, and greatly reduced phototoxicity (compared with single-photon confocals). The designated user group consists of 13 scientists who serve as PIs on 27 research projects funded by 8 different institutes of the NIH. Key projects demand capturing time-series of confocal images over large regions of interest in multiple focal planes labeled with two or more fluorophores in order to visualize movements at m/sec speeds. These capabilities are integral to the research programs of several of the participating investigators and will greatly benefit the research efforts of all designated users. Projects include studies of RNA transport in Drosophila embryos, kinesin-mediated movements in cultured cells, post-Golgi sorting and axonal transport in cultured neurons, pH changes and protein movements in hair cells, calcium dynamics in brain slices, trafficking of iron transport proteins in epithelial monolayers, lipid raft-associated ion channels in fibroblasts, and potassium channels in pancreatic islet cells. Laser scanning microscopes cannot begin to meet these demands. The requested instrument will be equipped with accessories to permit flexible use by a diverse group of investigators. It will be housed in OHSU's Live-Cell Imaging Facility, which currently serves a group of 35 registered users drawn from among the >200 research laboratories located on the main campus of OHSU. Use of the instrument will be overseen by Bruce Schnapp (Professor, Cell and Developmental Biology), Gary Banker (Senior Scientist, CROET), and a steering committee drawn from other departments. Together, Schnapp and Banker have more than 30 years of expertise with advanced imaging methods. User training and day-to-day operation of the instrument will be the responsibility of Dr. Stefanie Kaech, who has 10 years experience with confocal imaging and participated in beta-testing of the Ultraview while on the staff at the Friedrich Miescher Institute (Basel).

描述（由申请人提供）：本申请支持购买Perkin-Elmer Ultraview LCI共聚焦显微镜。这种新设计的显微镜，在Nipkow光盘技术的基础上，代表了活细胞共聚焦成像的重大进展。与使用激光扫描的显微镜相比，Ultraview能够更快地获取图像，实时共焦观察，并大大降低光毒性（与单光子共焦相比）。指定的用户组由13名科学家组成，他们担任NIH 8个不同研究所资助的27个研究项目的PI。关键项目需要在多个焦平面上捕获感兴趣的大区域上的共焦图像的时间序列，这些焦平面标记有两个或更多个荧光团，以便以m/sec的速度可视化运动。这些能力是几个参与研究者的研究计划的组成部分，将大大有利于所有指定用户的研究工作。项目包括研究果蝇胚胎中的RNA运输，培养细胞中驱动蛋白介导的运动，培养神经元中的高尔基体后分选和轴突运输，毛细胞中的pH变化和蛋白质运动，脑切片中的钙动力学，上皮单层中铁运输蛋白的运输，成纤维细胞中的脂筏相关离子通道和胰岛细胞中的钾通道。激光扫描显微镜无法开始满足这些要求。所要求的仪器将配备附件，以允许不同的研究人员灵活使用。它将被安置在OHSU的活细胞成像设施中，该设施目前为来自位于OHSU主校区的200多个研究实验室中的35个注册用户提供服务。该仪器的使用将由布鲁斯施纳普（教授，细胞和发育生物学），加里银行家（高级科学家，CROET）和一个指导委员会从其他部门。Schnapp和Banker在先进的成像方法方面拥有30多年的专业知识。用户培训和仪器的日常操作将由Stefanie Kaech博士负责，他拥有10年的共焦成像经验，并在Friedrich Miescher Institute（巴塞尔）工作期间参与了Ultraview的β测试。