MECHANISMS OF CHRONIC PROSTATITIS

描述 目前，人们对T的存在和作用几乎一无所知 慢性非细菌性疾病患者淋巴细胞、肥大细胞及其产物的研究 前列腺炎(NBCP)。因此，至关重要的是，系统地 在NBCP中对这些细胞及其产物进行表征以 填补我们对这种炎症性疾病知识的这一重大空白。因此，我们 建议：1)系统地表征T淋巴细胞亚群 NBCP和非NBCP患者(有关临床标准，请参阅患者群体 2)确定与CD4Th2相关的细胞因子，特别是IL3的作用 在促进NBCP肥大细胞的生长和分化方面，以及3) 确定肥大细胞及其产物在NBCP和非NBCP中的作用。 我们假设NBCP是一种前列腺炎性疾病 由特定T淋巴细胞亚群失衡所致。为了测试 在这一假设下，我们制定了以下具体目标：(1) 从体液中分离、鉴定和关联T细胞和T细胞克隆 (血液、尿液、前列腺液)和来自NBCP、非NBCP和正常的组织 (2)检测患者外周血中CD4Th1和CD4Th1的分布及相互关系 体液中的CD4Th2淋巴细胞和相关细胞因子(血液、尿液、 前列腺液)和来自NBCP、非NBCP和正常患者的组织；以及(3) 对肥大细胞和肥大细胞的分布进行表征和相互关联 NBCP、非NBCP和正常患者细胞相关因子的研究

DESCRIPTION Currently, virtually nothing is known about the existence and role of T lymphocytes, mast cells and their products in non bacterial chronic prostatitis (NBCP). Thus, it is essential that a systematic characterization of these cells and their products in NBCP be undertaken to fill this major gap in our knowledge of this inflammatory disease. Thus, we propose to: 1) systematically characterize T lymphocyte subpopulations in NBCP and non NBCP patients (for clinical criteria, see Patient Population below); 2) determine the role of CD4 Th2-related cytokines, particularly IL3 in promoting mast cell growth and differentiation in NBCP, and 3) to determine the role of mast cells and their products in NBCP and non NBCP. We hypothesize that NBCP is an inflammatory disease of the prostate that results from an imbalance of specific T-lymphocyte subpopulations. To test this hypothesis, we have developed the following specific aims: (1) to isolate, characterize and correlate T cells and T cell clones from fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patient; (2) to characterize and correlate the distribution of CD4 Th1 and CD4 Th2 lymphocytes and associated cytokines in fluids (blood, urine, prostate fluids) and tissue from NBCP, non NBCP and normal patients; and (3) to characterize and correlate the distribution of mast cells and mast cell-related factors in NBCP, non NBCP and normal patients.