CELL ASSENBLY OF BASEMENT MEMBRANE IN COCULTURES

培养物中基底膜的细胞组件

• 批准号: 2634589
• 负责人: QIHANG ZHANG
• 金额: $ 3.02万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-23 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2634589
• 关键词: basement membrane; epithelium; membrane biogenesis; mesenchyme; mixed tissue /cell culture

Basement membranes, cell associated heteropolymers, are specialized extracellular matrices and are important for development and maintenance of tissue functions. An in vitro system of epithelial cells and fibroblasts co-culture was used to study the role of cell surface receptors in the assembly of a sub-epithelial basement membrane. A basement membrane was formed only in co-culture although fibroblasts alone synthesize and secret most basement membrane components. When epithelial cells were cultured singly on a type I collagen gel containing basement membrane proteins (laminin, entactin/nidogen), these proteins diffused through the gel and were concentrated in the sub- epithelial region over several days suggesting that these components assembled on selected surface receptors or existing matrix components. Blocking integrin Beta-1 of epithelial cells by a monoclonal antibody caused dramatic effects on C2BBe cell adhesion, basement membrane formation and epithelial polarity. However, the amount of laminin and entactin/nidogen in antibody treated co-culture remained the same as that in control. The results suggest that the assembly of sub- epithelial basement membrane in vivo require interaction between cell surface receptors and matrix components.

基底膜，细胞相关的杂聚物， 细胞外基质和重要的发展和维护 组织功能。 上皮细胞的体外系统， 采用成纤维细胞共培养的方法研究细胞表面 受体在组装的一个亚上皮基底膜。 一 基底膜仅在共培养中形成，尽管成纤维细胞 单独合成和分泌大部分基底膜成分。 当 将上皮细胞单独培养在I型胶原凝胶上 含有基底膜蛋白（层粘连蛋白，巢蛋白/巢蛋白），这些 蛋白质扩散通过凝胶，并浓缩在亚微米级的凝胶中。 表明这些成分 组装在选定的表面受体或现有的基质组分上。 用单克隆抗体阻断上皮细胞整合素β 1 对C2BBe细胞的粘附、基底膜的形成、 形成和上皮极性。 然而，层粘连蛋白和 抗体处理的共培养物中的巢蛋白/巢蛋白保持相同， 一切尽在掌握 结果表明，组装的亚- 体内上皮基底膜需要细胞之间相互作用 表面受体和基质成分。