REGULATION OF PROLACTIN SECRETION AT THE LACTOTROPH

泌乳素泌乳素分泌的调节

• 批准号: 2701092
• 负责人: MARC Edward FREEMAN
• 金额: $ 21.74万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2701092
• 关键词: G protein; antisense nucleic acid; arachidonate; biological signal transduction; calcium; calcium channel; dopamine; dopamine receptor; eicosanoid metabolism; electrophysiology; fluorescence microscopy; hormone regulation /control mechanism; hypothalamic pituitary axis; laboratory rat; neurohormones; phospholipase A2; pituitary gland; prolactin; prolactin releasing /inhibiting factor; receptor coupling; secretion; tissue /cell culture; voltage /patch clamp

Prolactin (pRL) is a hormone which originates in cells of the anterior pituitary gland, lactotrophs, and plays multiple physiological roles in all mammals. Not only does it promote lactation in all mammals, but it also plays a role in maternal behavior, maintenance of pregnancy as well as osmoregulation and the immune response. Dopamine (DA) is a well established physiological inhibitor of PRL secretion. It originates in cells, neurons, within a specialized area of the brain, the hypothalamus, which sends projections that terminate upon the vasculature connecting the hypothalamus with the pituitary gland. It has recently been shown that DA is also a physiological stimulator of PRL secretion. The long term objective of this application is to characterize the mechanism whereby DA has opposing physiological effects on PRL secretion. Using measurements of PRL secretion as well as intracellular transduction events, the Specific Aims of this project will be to: (1) determine which DA receptors on the lactotroph are responsible for stimulation of PRL secretion, (2) determine which G-proteins are linked to stimulation versus inhibition of PRL secretion by DA, (3) determine if the intracellular lipids are involved in the stimulation of PRL secretion by DA and (4) determine the role of calcium in DA-stimulated PRL secretion. Since PRL has so many multi- faceted roles in the physiology of the organism, it is critical for purposes of possible therapeutic approaches to diseases involving PRL that all aspects of its physiological control be clarified. For example, both benign and malignant diseases of the mammary gland are approached by manipulating pituitary PRL secretion. Thorough knowledge of the control of PRL secretion will allow other approaches to its manipulation in disease states.

催乳素（PRL）是一种激素，起源于前额叶的细胞， 脑垂体，催乳素，并发挥多种生理作用， 所有哺乳动物它不仅能促进所有哺乳动物的泌乳， 也在母性行为中起作用， 作为免疫调节和免疫反应。多巴胺（DA）是一种 建立了PRL分泌的生理抑制剂。它起源于 细胞，神经元，在大脑的一个专门区域，下丘脑， 其发出终止于连接血管的突起， 下丘脑和脑下垂体最近的研究表明， 也是PRL分泌的生理刺激物。长期 本申请的目的是表征DA 对PRL分泌有相反的生理作用。通过测量 PRL分泌以及细胞内转导事件，特异性 本研究的目的是：（1）确定多巴胺受体的种类， 催乳素负责刺激催乳素分泌，（2）确定 哪些G蛋白与催乳素的刺激和抑制有关 （3）确定细胞内脂质是否参与DA的分泌， DA对PRL分泌的刺激作用;（4）确定 钙在DA刺激的PRL分泌中。由于PRL有这么多的多- 在生物体的生理学方面的作用，它是至关重要的， 涉及PRL的疾病的可能治疗方法的目的， 它的生理控制的各个方面都要弄清楚。例如， 乳腺良性和恶性疾病的处理， 控制垂体催乳素分泌全面了解控制 PRL分泌将允许其他方法在疾病中对其进行操纵 states.