METABOLISM OF HDL SUBPOPULATIONS--INFLUENCE OF DIET

描述：已知高密度脂蛋白浓度是 对评估冠心病风险很重要。 减少饮食 已知脂肪会降低HDL浓度，但对 对HDL亚组分分布影响。 在某些高血压病中， 状态，如高胆固醇血症，HDL浓度较低。 因此，在本发明中， 本申请的中心问题之一是饮食诱导的 HDL的降低将导致HDL亚组分的类似变化， 由于高脂血症而导致HDL低的受试者。 第一部分 建议，高脂血症和正常血脂受试者HDL亚组分 将进行研究，饮食诱导的HDL变化及其 将评价与HDL亚组分变化的关系。 在 第二部分建议，调制HDL的分布 HDL形成的亚组分和机制将使用 成纤维细胞和巨噬细胞。 这些后续研究将 双向定量电泳分离HDL优势 不经离心的亚级分。

DESCRIPTION: High density lipoprotein concentrations are known to be important in assessing coronary heart disease risk. Reduction in dietary fat is known to decrease HDL concentrations, but little was known regarding its influence on HDL subfraction distribution. In certain hyperlipidemic states, such as hypertriglyceridemia, HDL concentrations are lower. Thus, one of the central questions of this application is whether diet-induced reduction in HDL will result in similar changes in HDL subfractions compared to subjects who have low HDL due to hyperlipidemia. In the first part of the proposal, HDL subfractions in normolipidemic and hyperlipidemic subjects will be studied and the role of diet-induced HDL changes and their relationship to changes in HDL subfractions will be evaluated. In the second part of the proposal, modulation of the distribution of HDL subfractions and mechanism of HDL formation will be studied using fibroblasts and macrophages in culture. These later studies will take advantage of two-dimensional quantitative electrophoresis to isolate HDL subfractions without centrifugation.