INFECTIVITY OF PAUCIBACILLARY PULMONARY TUBERCULOSIS

描述 该FIRCA应用的父项目是一个分子流行病学 墨西哥恰帕斯州结核分枝杆菌研究。 的 研究人员正在使用分子基因分型方法来描述 多样性M.所有抗酸杆菌（AFB）涂片中的结核菌株 肺结核阳性病例。 结核病患者感染的集群 相同的菌株将在人口统计学、地理和 社会经济学术语。 生物，社会和行为因素的选择 集群将与非集群病例进行比较。 建立一个结核病委员会 分子流行病学实验室（DNA指纹、PCR和快速药物 敏感性测定）在国家诊断研究所 墨西哥城的流行病学是一个技术转让目标。 FIRCA研究的目的是应用分子流行病学 技术（分离株的IS6110基因分型），以评估 痰液显微镜检查未检出微生物学确诊的肺结核 （少杆菌）确实是感染他人的来源。 这将 能够重新评估假设AFB涂片阳性病例 有助于疾病传播。 由此产生的分子基因型将 进行分析，以确定结核病病例的集群，然后将其与 通过流行病学方法获得的病例中的传播事件。 本研究将在墨西哥普埃布拉州进行， 大部分在农村，在社会和经济上处于边缘地位。

DESCRIPTION The parent project for this FIRCA application is a molecular epidemiologic study of Mycobacterium tuberculosis in the state of Chiapas, Mexico. The investigators are using molecular genotyping methods to describe the diversity of M. tuberculosis strains from all acid-fast bacilli (AFB) smear positive cases of pulmonary TB. Clusters of TB patients infected with the same strain will be characterized in demographic, geographic, and socioeconomic terms. Biologic, social, and behavioral factors of selected clusters will be compared to non-clustered cases. The establishment of a TB molecular epidemiology lab (DNA fingerprinting, PCR and rapid drug susceptibility determination) in the Instituto Nacional de Diagnostico y Epidemiologicas in Mexico City is a technology transfer goal. The purpose of the FIRCA study is to apply molecular epidemiologic techniques (IS6110 genotyping of isolates) to evaluate whether persons with microbiologically confirmed pulmonary TB not detected by sputum microscopy (paucibacillary) truly are a source of infection to others. This would enable reassessment of the assumption that only AFB smear positive cases contribute to disease transmission. The resulting molecular genotypes will be analyzed to identify clusters of TB cases and will then be correlated with transmission events in the cases obtained by epidemiologic methods. This study will be conducted in the state of Puebla, Mexico, which is largely rural, and socially and economically marginal.