QUALITY CONTROL OF STUDIES OF OLIGOSACCHARIDE CHEMICAL ENZYMATIC SYNTHESIS

低聚糖能阻止细菌附着到 体外培养的上皮细胞--LNnT及其α 对2-1，3-和α2-6-唾液酸衍生物进行了检测 延缓肺炎链球菌肺炎病程的能力 在动物模型中预防鼻咽部的定植。 这些药物在气管内同时给药 细菌显著降低了肺炎球菌在肺中的负荷 并提供对菌血症的保护。几个 低聚糖是由多种化学物质组合而成的。 和酶促合成。这些样本改善了肺炎和 感染后24小时给予治疗性治疗的菌血症 已经成立了。鼻腔给药时，新糖偶联物 活性寡糖可防止鼻咽的定植 幼鼠的数量。除了体外抗粘连性能外， LNNTT直接作用于培养的肺上皮细胞株诱导 这样的变化，肺炎球菌的依从性被长期阻止 句号。这些活动鼓励了 低聚糖作为一类潜在的预防和治疗药物 治疗传染病的药物。微软资源的工作是 仔细检查合成的产物的序列和连接。 对制备的化合物的另一个担忧是，我们有一些 纯度评估。

Oligosaccharides that block the adherence of bacteria to epithelial cells in vitro-lacto-N-neotetraose (LNnT) and its alpha 2-1,3- and alpha 2-6-sialylated derivatives were tested for their abilities to attenuate the course of pneumococcal pneumonia and to prevent colonization of thenasopharynx in animal models. Intratracheal administration of these agents concurrently with bacteria dramatically decreased pneumococcal load in the lungs of rabbits and conferred protection from bacteremia. Several oligosaccharides were prepared by a combination of chemical andenzymatic synthesis. The samples ameliorated pneumonia and bacteremia when given therapeutically 24 h after infection was established. When administered intranasally, neoglycoconjugates of the active oligosaccharides prevented colonization of the nasopharynx of infant rats. In addition to in-vitro anti-adherence properties, LNnT acted directly on cultured lung epithelial cell lines to induce changes such that pneumococcal adherence was prevented for prolonged periods. These activities have encourage continued development of oligosaccharides as a class of potentially preventive and therapeutic agents for infectious diseases. The work with the MS Resource is to carefully check the products synthesized for sequence and linkage. Additional concern for the compounds prepared is that we have some assessment for purity.