UL33 GENE PRODUCT OF HSV1

描述在单纯疱疹病毒（HSV-1）感染细胞的细胞核内，复制的HSV-DNA形成头-尾多联体，其被切割成基因组长度的分子，随后易位到形成的衣壳中。与该建议相关的长期目标是定义HSV DNA切割和包装的分子机制。HSV基因UL 6、UL 15、UL 17、UL 28、UL 32和UL 33是衣壳组装所必需的，但已被证明是病毒DNA切割和包装所必需的。UL 33被导入受感染细胞的细胞核中。输入由晚期基因产物介导。本提案的总体目的是通过以下方式表征UL 33蛋白在HSV-1包装体中的作用：1）鉴定病毒UL 33核重要性所需的基因产物，和2）鉴定UL 33与其他HSV蛋白之间蛋白质-蛋白质相互作用所需的关键蛋白质结构域。我们对UL 33基因产物进行了表位标记，并开发了一种核输入测定法，以检查缺乏特异性切割/包装基因的病毒对UL 33蛋白核输入的影响。

DESCRIPTION Within the nuclei of herpes simplex virus (HSV-1) infected cells, replicating HSV-DNA forms head-to-tail concatemers which are cleaved to genome length molecules and subsequently translocated into performed capsids. The long term objective related to this proposal is the definition of the molecular mechanisms of HSV DNA cleavage and packaging. The HSV genes UL6, UL15, UL17, UL28, UL32, and UL33 are dispensable for capsid assembly, but have been shown to be necessary for viral DNA cleavage and packaging. UL33 is imported into the nucleus in infected cells. Importation is mediated by late gene product(s). The overall aim of this proposal is to characterize the role of UL33 protein in the HSV-1 packasome by 1) Identifying gene products required for viral UL33 nuclear important and 2) Identifying the critical protein domains necessary for protein-protein interactions between UL33 and other HSV proteins. We have eptiopically-tagged the UL33 gene product and developed a nuclear importation assay examining the effect of viruses lacking specific cleavage/packaging genes on nuclear importation of UL33 protein.