UL33 GENE PRODUCT OF HSV1

描述在单纯疱疹病毒(HSV-1)感染细胞的细胞核内，复制的HSV-DNA形成头尾相连的链接体，这些链接体被裂解成基因组长度的分子，然后转移到执行的衣壳中。与这一提议相关的长期目标是定义HSV DNA切割和包装的分子机制。单纯疱疹病毒UL6、UL15、UL17、UL28、UL32和UL33基因对于衣壳组装是不必要的，但已被证明是病毒DNA切割和包装所必需的。UL33在感染细胞中被输入到细胞核中。进口是由晚期基因产物介导的(S)。这项建议的总体目的是通过1)鉴定病毒UL33核重要所需的基因产物，以及2)鉴定UL33与其他HSV蛋白之间蛋白质-蛋白质相互作用所必需的关键蛋白结构域，来表征UL33蛋白在HSV-1包装体内的作用。我们已经对UL33基因产物进行了表位标记，并建立了一种核导入试验，以检测缺乏特定切割/包装基因的病毒对UL33蛋白核导入的影响。

DESCRIPTION Within the nuclei of herpes simplex virus (HSV-1) infected cells, replicating HSV-DNA forms head-to-tail concatemers which are cleaved to genome length molecules and subsequently translocated into performed capsids. The long term objective related to this proposal is the definition of the molecular mechanisms of HSV DNA cleavage and packaging. The HSV genes UL6, UL15, UL17, UL28, UL32, and UL33 are dispensable for capsid assembly, but have been shown to be necessary for viral DNA cleavage and packaging. UL33 is imported into the nucleus in infected cells. Importation is mediated by late gene product(s). The overall aim of this proposal is to characterize the role of UL33 protein in the HSV-1 packasome by 1) Identifying gene products required for viral UL33 nuclear important and 2) Identifying the critical protein domains necessary for protein-protein interactions between UL33 and other HSV proteins. We have eptiopically-tagged the UL33 gene product and developed a nuclear importation assay examining the effect of viruses lacking specific cleavage/packaging genes on nuclear importation of UL33 protein.