TH1 CELLS AND LUNG INJURY

本研究项目的长期目标是了解淋巴细胞亚群在肺损伤产生中的作用，特别是当它与骨髓移植人群有关时。该项目的具体目标是深入了解淋巴细胞归巢到肺部以及这些细胞产生局部炎症反应的机制。为了实现这些目标，我们将使用的研究设计将涉及对Th1细胞克隆的研究，这是一种已知在移植物抗宿主病的产生中至关重要的细胞类型。我们将尝试通过单克隆抗体、特异性化学抑制剂和缺乏粘附分子的转基因小鼠抑制白细胞粘附来阻断这些细胞在肺中的定位。我们将通过研究与白细胞募集相关的炎症介质（趋化因子）的阐述来研究这些细胞产生局部反应的机制。

The long-term objective of this research project is to understand the role of a subpopulation of lymphocytes in the generation of lung injury, particularly as it pertains to the bone marrow transplant population. The specific aims of this project are to gain insight into lymphocyte homing to lung and the mechanisms by which these cells generate an inflammatory response localized to lung. The research design that we will use to achieve these aims will involve the study of a Th1 cell clone, a cell type known to be crucial in the generation of graft versus host disease. We will attempt to block localization of these cells in lung by inhibiting leukocyte adhesion with monoclonal antibodies, specific chemical inhibitors and transgenic mice deficient in adhesion molecules. We will investigate the mechanisms by which these cells generate a localized response by studying the elaboration of inflammatory mediators (chemokines) that have relevance to leukocyte recruitment.