BACTERIAL ADP-RIBOSYLTRANSFERASES--A ROLE IN SYMBIOSIS

产品描述费氏弧菌-Euprymna piglopes光器官共生体证实了典型的动物细菌相互作用-宿主组织的稳定、非致病性细菌定植，动物及其微生物群能够相互识别、发出信号并在发育上相互影响。光器官组织的几种发育变化仅发生在感染费氏弧菌的动物中，然而细菌定植和诱导发育的机制尚不清楚。本研究的目的是研究费氏弧菌输出的两种诺埃尔的ADP-核糖基转移酶（ADPrs）HvnA和HvnB在该细菌在大肠杆菌中定殖和引发发育变化的能力中的作用。我是说... ADPR共价修饰蛋白质，内源性ADPR调节重要的细胞过程，而已知的排泄细菌ADPR在致病相互作用中对宿主产生毒性作用。因此，我们假设ADPrs在这种共生中起作用。我们建议：（i）克隆和表征鱿鱼共生体中的hvnA和hvnB操纵子，（ii）确定hvnA和hvnB基因是否在E.（iii）检测缺失hvnA和/或hvnB的突变体在E. HvnA和HvnB在光器官内的免疫定位和（v）确定特定宿主蛋白质是否被HvnA和HvnB ADP核糖基化。

DESCRIPTION The Vibrio fischeri-Euprymna scolopes light organ symbiosis exemplifies typical animal bacterial interactions-stable, non-pathogenic bacterial colonization of host tissue with both the animal and its microflora able to recognize, signal, and developmentally influence one another. Several developmental changes in light organ tissue occur only in animals infected with V. fischeri, however the mechanisms underpinning bacterial colonization and induction of development are unknown. The aim of this proposal is to investigate the role of two noel ADP-ribosyltransferases (ADPrs) exported by V. fischeri, HvnA and HvnB, in this bacterium's ability to colonize and trigger developmental changes in E. scolopes. ADPRs covalently modify proteins, and endogenous ones regulate important cellular processes while known excreted bacterial ADPrs exert toxic effects on hosts in pathogenic interactions. We therefore hypothesize that ADPrs play a role in this symbiosis We propose to: (i) clone and characterize the hvnA and hvnB operons from a squid symbion, (ii) determine whether hvnA and hvnB genes are expressed in the E. scolopes light organ, (iii) test the ability of mutants lacking hvnA and/or hvnB to colonize and trigger development in E. scolopes, (iv) localize HvnA and HvnB within the light organ immunologically and (v) determine whether specific host proteins are ADP-ribosylated by HvnA and HvnB.