REGULATION AND EVOLUTION OF DROSOPHILA LEG PATTERNING

果蝇腿部图案的调节和进化

• 批准号: 2857526
• 负责人: JOHN R TRUE
• 金额: $ 3.57万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2857526
• 关键词: Drosophilidae; developmental genetics; embryogenesis; genetically modified animals; invertebrate embryology; limbs; molecular cloning

Animals are adorned with structural patterns that arise in highly specific places. In order to assure the consistent positioning of these structures, the developmental system must use dependable sets of regulatory factors specifying where the expression of particular genes must be activated or repressed. The developmental or genetic "address" conveyed by these regulators integrates various signals corresponding to segment identity, segment polarity, proximal-distal axis position, and sex. In the proposed experiments, the function of this developmental circuitry in specifying Drosophila melanogaster leg bristle patterns will be explored. Insect bristles function as peripheral sense organs and are a powerful model system for studying pattern formation in cellular fields. First, the expression patterns of major developmental address regulators will be characterized in the leg. Second, enhancer regions of the achaete-scute complex (AS-C) of proneural genes will be characterized as to their responses to developmental address regulators. Third, structures of enhancers needed for formation of specific leg bristle motifs will be investigated by identifying binding sites for known address-regulating proteins. Finally, the evolution of this developmental circuitry will be examined by cloning and characterizing AS-C enhancers from Drosophila species with divergent leg bristle patterns and examining their function in transgenic D. melanogaster lines. These studies will illuminate mechanisms by which position-specific mixtures of regulatory proteins are interpreted during pattern formation, as well as how these mechanisms have evolved to produce diversity among iterated appendages in a single species and among homologous appendages in different species.

动物装饰有高度出现的结构模式 特定的地方。 为了确保这些的一致定位 结构，开发系统必须使用可靠的集合 指定特定基因表达的调节因素 必须被激活或压抑。 发展或遗传“地址” 这些监管机构传达的各种信号与 段身份，段极性，近端轴位置和 性别。在拟议的实验中，这种发展的功能 指定果蝇的电路Melanogaster腿毛皮模式将 被探索。昆虫刷毛的作用充当外围感官，是 一个强大的模型系统，用于研究细胞中的模式形成 字段。首先，主要发育地址的表达方式 调节器将在腿部进行表征。其次，增强子区域 将表征赤乳基因的Achaete-Scute复合物（AS-C） 关于他们对发育地址监管机构的反应。第三， 形成特定腿部刷毛所需的增强剂结构 将通过识别已知的结合位点来研究图案 地址调节蛋白。最后，这种发展的发展 电路将通过克隆和表征AS-C增强剂来检查 来自具有不同腿部毛皮图案的果蝇物种和检查 它们在转基因D. Melanogaster线中的功能。这些研究会 照明调节的特定位置特异性混合物的机制 蛋白质在模式形成过程中被解释，以及如何解释 机制已经发展为在迭代的附属物中产生多样性 一个物种以及不同物种的同源附属物。