DEVELOPMENT OF CAROTID BODY CHEMOTRANSDUCTION

颈动脉体化学传导的发展

• 批准号: 6030362
• 负责人: ESTELLE B. GAUDA
• 金额: $ 13.61万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6030362
• 关键词: carotid body; chemoreceptors; gene expression; growth /development; histochemistry /cytochemistry; in situ hybridization; inhibitor /antagonist; neural transmission

DESCRIPTION: It is the purpose of this 5-year program to investigate molecular mechanisms involved in arterial chemoreception during postnatal development. In particular, the hypothesis that the net result of neurotransmission in arterial chemoreceptors is inhibitory and, as such, produces unstable ventilation will be tested. It is further proposed that the lack of the induction of immediate-early genes in cells of the chemoreceptor neural pathway (i.e., carotid bodies, petrosal ganglia, NTS and hypoglossal) by excitatory stimuli will serve as a marker of this inhibition. The applicant will use in situ hybridization histochemistry: a) to characterize the distribution of neurotransmitters and their receptors in cells of the carotid bodies and petrosal ganglia; b) to determine the effect of development on the induction of the immediate-early gene in cells of the chemoreceptor neural pathways; and c) to determine the effects of acute and chronic receptor agonists and antagonists on gene expression in response to peripheral and central chemoreceptor activation (via hypoxia).

描述：这是这个5年计划的目的是调查 动脉化学感受的分子机制 产后发育 特别是，假设净结果 动脉化学感受器的神经传递是抑制性的， 这样，产生不稳定的通风将被测试。 进一步 提出，缺乏诱导的立即早期基因， 化学感受器神经通路的细胞（即，颈动脉体， 岩神经节，NTS和舌下神经）的兴奋性刺激将服务 作为这种抑制的标志。 申请人将在原地使用 杂交组织化学：a）表征 颈动脉体细胞中的神经递质及其受体， B）以确定发育对神经节的影响; 化学感受器细胞中即刻早期基因的诱导 神经通路;以及c）确定急性和慢性 受体激动剂和拮抗剂对基因表达的影响 外周和中枢化学感受器激活（通过缺氧）。