Biosynthetic LEGO: enzymatic redesign to produce new vancomycin analogues

生物合成乐高：酶法重新设计以生产新的万古霉素类似物

• 批准号: DP190101272
• 负责人: Prof Max Cryle
• 金额: $ 33.7万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190101272
• 关键词: Biosynthetic; LEGO; enzymatic; redesign; produce

This project aims to uncover the reengineering potential of the biosynthetic machinery that produces glycopeptide antibiotics by advancing our understanding of how the core peptide production line functions. Natural product biosynthesis often produces complex peptide structures, with one important example being the glycopeptide antibiotics. This project expects to generate new knowledge about enzymatic peptide biosynthesis using a highly interdisciplinary approach and previously developed tools. The anticipated outcomes of this project will be an enhanced understanding of how such complex peptide biosynthesis is performed, which is knowledge vital for future efforts to reengineer such biosynthetic peptide assembly lines as a series of modular LEGO blocks to produce new bioactive peptides.

该项目旨在通过推进我们对核心肽生产线如何运作的理解，揭示生产糖肽抗生素的生物合成机器的重组潜力。天然产物的生物合成通常会产生复杂的肽结构，其中一个重要的例子是糖肽抗生素。该项目期望利用高度跨学科的方法和先前开发的工具产生关于酶促肽生物合成的新知识。该项目的预期结果将是加强对如何进行这种复杂肽生物合成的理解，这对于未来重新设计这种生物合成肽装配线作为一系列模块化乐高积木来生产新的生物活性肽至关重要。