Biosynthetic LEGO: enzymatic redesign to produce new vancomycin analogues

该项目旨在通过推进我们对核心肽生产线功能的理解，揭示生产糖肽抗生素的生物合成机制的再造潜力。天然产物的生物合成通常产生复杂的肽结构，其中一个重要的例子是糖肽抗生素。该项目希望使用高度跨学科的方法和先前开发的工具产生关于酶促肽生物合成的新知识。该项目的预期成果将是增强对这种复杂肽生物合成如何进行的理解，这对于未来将这种生物合成肽组装线重新设计为一系列模块化乐高积木以生产新的生物活性肽的努力至关重要。

This project aims to uncover the reengineering potential of the biosynthetic machinery that produces glycopeptide antibiotics by advancing our understanding of how the core peptide production line functions. Natural product biosynthesis often produces complex peptide structures, with one important example being the glycopeptide antibiotics. This project expects to generate new knowledge about enzymatic peptide biosynthesis using a highly interdisciplinary approach and previously developed tools. The anticipated outcomes of this project will be an enhanced understanding of how such complex peptide biosynthesis is performed, which is knowledge vital for future efforts to reengineer such biosynthetic peptide assembly lines as a series of modular LEGO blocks to produce new bioactive peptides.