RNA/PROTEIN INTERACTIONS REGULATING MRNA STABILITY

RNA/蛋白质相互作用调节 mRNA 稳定性

• 批准号: 2701221
• 负责人: MEGERDITCH KILEDJIAN
• 金额: $ 14.84万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2701221
• 关键词: RNA binding protein; RNase protection assay; alpha globulin; chemical stability; gel mobility shift assay; gene expression; genetic regulation; genetic regulatory element; genetic translation; high performance liquid chromatography; immunoprecipitation; laboratory mouse; messenger RNA; monoclonal antibody; polymerase chain reaction; protein purification; protein sequence; protein structure function; ribonucleoproteins; tissue /cell culture; transcription factor; yeast two hybrid system

DESCRIPTION (Adapted from investigators's abstract) The overall objective of this proposal is to further the understanding of the determinants that regulate globin mRNA stability. This proposal has three main aims: 1) to identify all the alpha complex proteins both biochemically and genetically; 2) to functionally characterize the alpha-globin mRNA stability mechanisms by using an alpha-complex dependent in vitro mRNA decay system; and 3) to identify additional mRNA(s) bound by and possibly regulated by the alpha complex.

描述（改编自研究者摘要） 这一建议是为了进一步了解决定因素， 调节珠蛋白mRNA的稳定性。 这项建议有三个主要目的：（1） 从生物化学和遗传学上鉴定所有的α复合体蛋白; 2)从功能上表征α-珠蛋白mRNA稳定性机制 通过使用α-复合物依赖性体外mRNA衰变系统;和3） 鉴定与α-半乳糖苷酶结合并可能受其调节的其他mRNA 复杂.