Regulation of Mammalian mRNA Decay
哺乳动物 mRNA 衰变的调控
基本信息
- 批准号:10350594
- 负责人:
- 金额:$ 37.98万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2004
- 资助国家:美国
- 起止时间:2004-09-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:AddressAdenineAffectAffinityBindingCell ExtractsCell physiologyCellsCellular StressComplexDNADiphosphatesDiseaseElementsEnzymesEukaryotaFlavin-Adenine DinucleotideFlavinsFoundationsGene ExpressionGenetic TranscriptionGenetic TranslationGuanosineHomeostasisHumanHydrolaseIn VitroKineticsLinkMalignant NeoplasmsMammalian CellMediatingMessenger RNAMetabolismMinorMitochondriaMolecularNADHNatureNicotinamide adenine dinucleotideNucleotidesOxidation-ReductionPharmacologyPhysiologicalPost-Transcriptional RegulationPrevalenceProteinsProto-OncogenesRNARNA Cap-Binding ProteinsRNA CapsRNA DecayRNA StabilityRNA metabolismRegulationRoleStressTranslationsWorkbaseclinical phenotypecofactorexosomegenetic informationinsightmRNA DecaymRNA StabilitymRNA cappingnovelnovel strategiesnucleasespleen exonuclease
项目摘要
Project Summary
The control of mRNA stability is a critical determinant in the post-transcriptional regulation of eukaryotic
gene expression. Even minor alterations in mRNA stability can have profound consequences and may
manifest as clinical phenotypes as illustrated by the ability of aberrantly expressed proto-oncogenes
that can give rise to malignancies. Eukaryotic mRNAs are generally thought to possess an N7 methyl
guanosine (m7G) cap at their 5¢ end to promote their stability and translation. However, our recent
demonstration that mammalian mRNAs can also carry a 5´-end nicotinamide adenine dinucleotide
(NAD) cap that in contrast to the m7G cap promotes mRNA decay, provides a new paradigm for mRNA
5´ end processing and the contribution of nucleotide metabolites in mRNA turnover. We now
demonstrate that the redox state of NAD can also modulate 3´ RNA decay with free NAD functioning
as a cofactor to enhance RNA decay and potentially providing a link to cellular energetics. Moreover,
flavin adenine diphosphate (FAD) can also serve as a 5´ cap on mammalian RNAs with Nudt16 and
DXO hydrolases functioning as proteins that can remove the FAD cap (deFADding) in vitro. We will
build on these novel findings throughout this proposal within three specific aims. The first will address
the functional role of free NAD on the control of 3´ end RNA decay in vitro and delineate the molecular
mechanism involved in its stimulation of decay. The second will deduce changes in mRNA decay as a
consequence of altered NAD levels in cells and assess the regulatory role imparted by stress conditions
in modulating RNA decay through the control of NAD levels. In the last aim, we establish FAD cap as
an alternative RNA cap, identify FAD-capped RNAs and decipher the role of FAD caps and the
deFADding enzymes in cells. Collectively, the proposed studies will provide insight into a heretofore
unknown fundamental post-transcriptional regulatory mechanism and will provide the framework for
potential novel avenues to control gene expression in normal and disease states.
项目摘要
mRNA稳定性的控制是真核生物转录后调控的关键因素,
基因表达。即使是mRNA稳定性的微小改变也会产生深远的影响,
表现为临床表型,如异常表达的原癌基因
会导致恶性肿瘤真核生物的mRNA通常被认为具有N7甲基,
鸟苷(m7G)帽在其5 ′端,以促进其稳定性和翻译。然而,我们最近
证明哺乳动物mRNA也可以携带5 ′端烟酰胺腺嘌呤二核苷酸,
(NAD)与m7G帽相反,m7G帽促进mRNA衰变,提供了一种新的mRNA
5 ′末端加工和核苷酸代谢物在mRNA周转中的贡献。我们现在
证明NAD的氧化还原状态也可以调节3 ′ RNA衰变与游离NAD功能
作为一种辅助因子来增强RNA衰变,并可能提供与细胞能量学的联系。此外,委员会认为,
黄素腺嘌呤二磷酸(FAD)也可以作为哺乳动物RNA上的5 ′帽,
DXO水解酶作为蛋白质发挥功能,可在体外去除FAD帽(deFADding)。我们将
在三个具体目标下,在整个提案中以这些新的发现为基础。第一个将解决
游离NAD在体外控制3 ′端RNA衰变中的功能作用,并描述了其分子机制。
在其刺激腐烂的机制。第二个将推导出mRNA衰变的变化,
细胞中NAD水平改变的结果,并评估应激条件赋予的调节作用
通过控制NAD水平来调节RNA衰变。在最后一个目标中,我们将FAD上限设置为
一个替代的RNA帽,鉴定FAD加帽的RNA,并破译FAD帽的作用,
细胞中的去脂肪酶。总的来说,拟议的研究将提供一个迄今为止的洞察力。
未知的基本转录后调控机制,并将提供框架,
控制正常和疾病状态下基因表达的潜在新途径。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MEGERDITCH KILEDJIAN其他文献
MEGERDITCH KILEDJIAN的其他文献
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{{ truncateString('MEGERDITCH KILEDJIAN', 18)}}的其他基金
Eukaryotic RNA NAD capping and deNADding
真核 RNA NAD 加帽和 deNADding
- 批准号:
10443996 - 财政年份:2018
- 资助金额:
$ 37.98万 - 项目类别:
Eukaryotic RNA NAD capping and deNADding
真核 RNA NAD 加帽和 deNADding
- 批准号:
10797880 - 财政年份:2018
- 资助金额:
$ 37.98万 - 项目类别:
Eukaryotic RNA NAD capping and deNADding
真核 RNA NAD 加帽和 deNADding
- 批准号:
10622526 - 财政年份:2018
- 资助金额:
$ 37.98万 - 项目类别:
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