ENVIRONMENTAL INFLUENCES ON RABBIT IGH DIVERSITY

环境对兔子 IGH 多样性的影响

• 批准号: 2590836
• 负责人: DENNIS K LANNING
• 金额: $ 2.86万
• 依托单位: LOYOLA UNIVERSITY CHICAGO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-11-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2590836
• 关键词: animal food; antibody formation; bacterial cytopathogenic effect; breast feeding; gene environment interaction; germ free condition; gut associated lymphoid tissue; immunogenetics; immunoglobulin genes; laboratory rabbit; microorganism immunology; milk; mucosal immunity; newborn animals; nutrition related tag; polymerase chain reaction

The goal of this project is to identify the external factor(s) which stimulate VDJ gene diversification of the rabbit antibody repertoire. The research design is based on the fact that rabbits isolated from normal colony flora and fed sterile milk formula and autoclaved solid food diversify their antibody to a much lesser extent than age-matched normal rabbits. Groups of these rabbits will be exposed differentially to normal colony flora, mother's milk, and food sterilized by alternate methods to identify the external factors(s) required for stimulation of rabbit VDJ diversification. Identifying this factor and the mechanism by which it mediates its effect would be a significant contribution to the field with potential implications for antibody repertoire development in humans.

这个项目的目标是找出外部因素(S)， 刺激兔抗体库VDJ基因的多样化。这个 研究设计是基于这样一个事实，即兔与正常动物分离 菌落菌群和喂养无菌奶粉和高压灭菌固体食物 使他们的抗体多样化到比年龄匹配的正常人小得多的程度 兔子。这些组的兔子将被区别地暴露在正常的环境中 菌落菌群、母乳和以其他方法灭菌的食物 确定刺激兔VDJ所需的外部因素(S) 多元化。确定这一因素以及它的机制 调解其影响将是对该领域的重大贡献 对人类抗体谱系发展的潜在影响。