CNS EFFICACY OF ANTIOXIDANTS FROM PLANTS

描述（改编自申请人的摘要）：中枢神经系统 中枢神经系统（CNS）似乎特别容易受到氧化应激的影响， 被认为是神经退化的主要因素之一。 的 引起氧化应激的活性氧（ROS）已经被 与几种CNS功能障碍的病因学有关，包括 帕金森病、亨廷顿病、阿尔茨海默病和老年痴呆症 个脑袋ROS的形成和扩散可能被延迟或抑制， 抗氧化剂可以是内源性或外源性来源的。 一 许多从植物中提取的物质已被证明具有 显著抗氧化活性，因此具有潜在的直接或 间接应用于治疗神经退行性疾病。 在 在我们项目的第一阶段，我们已经开发了半制备性的方法， 提取、定量测定和体外生物测定 五种计划抗氧化剂，即，胡萝卜中的β-胡萝卜素， 欧芹，番茄红素形成番茄酱，姜黄素和姜黄素来自 姜黄。 在计划的第二阶段，我们建议行程以下事项 问题：1）制定每种药物的商业制备程序 这些化合物的纯形式和良好的产率，使用最少的 昂贵的仪器和技术; 2）扩展 生物活性的个人和混合物（鸡尾酒），这些 抗氧化剂，以补充在体外条件 第一阶段已经开发的方法; 3）开发具体的 抗叶黄素、姜黄素和姜黄素的单克隆抗体。 拟定商业应用：不可用

DESCRIPTION (Adapted from Applicant's Abstract): The central nervous system (CNS) appears to be especially vulnerable to oxidative stress which is believed to be one of the major factors in neural degeneration. The reactive oxygen species (ROS) which cause the oxidative stress, have been implicated in the etiology of several CNS malfunctions, including Parkinsonism, Huntington's Disease, Alzheimer's Disease and aging of brain. The formation and spread of ROS may be delayed or inhibited by antioxidants which may be of either endogenous or exogenous origin. A number of substances derived from plants have been shown to possess significant antioxidant activity and, therefore, have potential direct or indirect applications in the treatment of neurodegenerative diseases. In Phase I of our project, we have developed methods for the semipreparative extraction, quantitative determination and in vitro biological assays of five plan antioxidants, viz., beta-carotene from carrots, lutein from parsley, lycopene form tomato paste, and curcumin and turmerin from turmeric. In Phase II of the project, we propose to address the following issues: 1) to develop procedures for the commercial preparation of each of these compounds in pure form and in good yield, using the least expensive instrumentation and technology; 2) to extend the assay of biological activities of individual and mixtures (cocktails) of these antioxidants to in vivo conditions so as to complement the in vitro methods already developed in Phase I; and 3) to develop specific monoclonal antibodies against lutein, curcumin and turmerin. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE