PROTEIN/WATER COHERENCE EFFECTS IN NMR AND IMAGING

描述：这是一份修订后的续订提案。总体目标 是开发梯度和射频脉冲整形的应用 用于生物分子的核磁共振波谱，特别是在 浓缩性溶剂。重点是分子间多量子。 (ImQ)蛋白质和水之间的连贯性和相声。这两个 关于稀溶液的理论和实验工作将继续进行 水中的生物分子。具体地说，活体成像和光谱 工作将延长，目标是增强图像对比度和 验证提出的功能磁共振成像机制。高分辨率核磁共振在 将使用产生不均匀磁场的磁体来追求高场 (例如，NHMFL现有的27T和拟议的30T磁铁)。工作于 通过溶质-溶剂相干转移的小信号放大也将被 以革命性的方式追求低伽马或 在核磁共振中检测到低浓度的物种。IMQ的抑制 将探索TOCSY等序列中的相干性，以产生更好的 版本。

DESCRIPTION: This is a revised continuation proposal. The broad objectives are to develop applications of gradients and radiofrequency pulse shaping for nuclear resonance spectroscopy of biomolecules, particularly in concentrated solvents. The focus is on intermolecular multiple-quantum (iMQ) coherences and crosspeaks between proteins and water. Both the theoretical and experimental work will be continued on dilute solution of biomolecules in water. Specifically, the in vivo imaging and spectroscopy work will be extended with the aims of enhancing image contrast and validating proposed mechanisms for functional MRI. High resolution NMR at high fields will be pursued using magnets which give inhomogeneous field (e.g. the existing 27T and proposed 30T magnets at the NHMFL). Work on small-signal amplification by solute-solvent coherence transfer will also be pursued with the view of revolutionizing the way in which low-gamma or low-concentration species are detected in NMR. Suppression of iMQ coherences in sequences such TOCSY will be explored to produce improved versions.