IDENTIFICATION OF BACTERIA DIRECT FROM BLOOD CULTURE

直接从血培养中鉴定细菌

• 批准号: 6014520
• 负责人: MYRON SASSER
• 金额: $ 9.45万
• 依托单位: MICROBIAL ID, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-06 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6014520
• 关键词: bacterial disease; communicable disease diagnosis; computer program /software; density gradient ultracentrifugation; diagnosis design /evaluation; fatty acids; gas chromatography; nosocomial infections; physical separation; rapid diagnosis; serology /serodiagnosis; technology /technique development

Rapid identification of bacteria direct from a blood culture bottle would speed patient treatment and recovery and greatly reduce hospital care costs. The current procedure of subculturing typically takes from 24 to 48 hours to obtain bacterial identifications. Gas chromatographic analysis of bacterial fatty acids has promise as a rapid and inexpensive identification technique if the system were made more sensitive and if impact of the fatty acid composition of the blood could be minimized. Splitless injection of the entire fatty acid extract and use of an electron capture detector may increase the chromatographic sensitivity by a factor of ca. 6,000,000X. Physical separation of bacteria from blood components by gradient centrifugation and/or filtration and the use of software techniques for reducing the impact of blood fatty acid components will be researched. New algorithms for naming bacteria and for dealing with polymicrobic cultures will be evaluated. By producing identification more rapidly than current techniques, the proposed technique could save ca. $1,000 per patient day of care costs where blood cultures are taken. By speeding treatment and recovery, this technique will also reduce patient suffering and aid in reducing nosocomial infections. PROPOSED COMMERCIAL APPLICATIONS: Identification of bacteria direct from a blood culture bottle will save at least one day by assuring speedy treatment of patients with septicemia thereby reducing medical care costs. MIDI will sell the software, gas chromatograph and consumables related to this application and will develop other applications based on the technique.

直接从血液培养瓶中快速鉴定细菌将加快患者的治疗和康复，并极大地降低医院护理成本。目前的继代培养程序通常需要24到48小时才能获得细菌鉴定。如果系统变得更灵敏，如果能将血液中脂肪酸组成的影响降至最低，细菌脂肪酸的气相色谱分析有望成为一种快速且廉价的识别技术。不分流注射整个脂肪酸提取物并使用电子捕获检测器可以将色谱灵敏度提高约6,000,000倍。将研究通过梯度离心和/或过滤从血液成分中物理分离细菌，以及使用软件技术减少血液脂肪酸成分的影响。将评估为细菌命名和处理多菌种培养的新算法。通过比目前的技术更快地产生身份证明，拟议的技术可以在进行血液培养的地方为患者节省约1,000美元的每日护理费用。通过加快治疗和康复，这项技术还将减少患者的痛苦，并有助于减少医院感染。拟议的商业应用：直接从血液培养瓶中鉴定细菌将确保败血症患者的快速治疗，从而降低医疗成本，从而至少节省一天的时间。MIDI将销售与这一应用程序相关的软件、气相色谱仪和消耗品，并将基于该技术开发其他应用程序。