RAPID INEXPENSIVE ASSAY FOR TB AND OTHER MYCOBACTERIA

结核病和其他分枝杆菌的快速、廉价检测

• 批准号: 2005779
• 负责人: MYRON SASSER
• 金额: $ 9.5万
• 依托单位: MICROBIAL ID, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1998-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2005779
• 关键词: Mycobacterium; clinical chemistry; communicable disease diagnosis; hydroxy fatty acid; liquid chromatography; method development; spectrometry; sputum; tuberculosis

Due to an increasing incidence of tuberculosis in the United States and the appearance of multiple drug resistant strains, a rapid and inexpensive test is needed to diagnose the disease. The standard PPD tuberculin test often fails in HIV infected (eg. drug using) subjects and may be ineffective in malnourished individuals or in those previously vaccinated with Mycobacterium bovis BCG. Identification by biochemical tests may require weeks and DNA techniques are very costly and usually identify a single species. High performance liquid chromatographic analysis of mycolic acids of mycobacteria provides inexpensive identification of all clinically relevant species, but has the serious disadvantage of requiring cultures grown for weeks. The proposed research will test the feasibility of using supercritical fluid chromatography (SFC) coupled with the very sensitive electron capture detector (ECD) for analysis of mycolic acids. When driven by computer software which automates analysis and performs pattern recognition comparisons to a stored database, the system should identify TB and other mycobacteria directly from sputum without culturing. The SFC-ECD system should approach the sensitivity of the DNA probes and PCR tests with the advantage of identifying all clinically relevant mycobacteria at a small fraction of the cost of the DNA techniques.

由于美国结核病发病率不断上升 和多重耐药菌株的出现，一种快速和 诊断这种疾病需要进行廉价的检测。标准 PPD结核菌素试验在感染HIV的患者中经常失败(例如，吸毒) 受试者，可能对营养不良的个人或 那些以前接种过牛分枝杆菌卡介苗的人。 通过生化测试进行鉴定可能需要数周时间和DNA 技术非常昂贵，通常识别单一物种。 霉菌的高效液相色谱分析 分枝杆菌的酸提供了廉价的所有 临床上相关的物种，但有严重的缺点 需要培养数周。拟议的研究将 超临界流体色谱柱的可行性试验 (SFC)与非常灵敏的电子捕获探测器相结合 (ECD)用于真菌酸的分析。当由计算机驱动时 自动化分析和执行模式的软件 与存储的数据库进行识别比较，系统应该 直接从痰中鉴定结核和其他分枝杆菌 培养。SFC-ECD系统应接近灵敏度 DNA探针和聚合酶链式反应检测的优势 用一小部分鉴定所有临床相关的分枝杆菌 DNA技术的成本。